Mvda is required for zebrafish early development

Abstract Background: The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoin- flammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the huma...

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Autores principales:	Wong,Wenghong, Huang,Yike, Wu,Zhuanbin, Kong,Yu, Luan,Jing, Zhang,Qiaoan, Pan,Jiewen, Yan,Kexiang, Zhang,Zhenghua
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	Mvda Mevalonate pathway Zebrafish Epidermis Angiogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100217
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spelling	oai:scielo:S0716-976020210001002172021-06-30Mvda is required for zebrafish early developmentWong,WenghongHuang,YikeWu,ZhuanbinKong,YuLuan,JingZhang,QiaoanPan,JiewenYan,KexiangZhang,Zhenghua Mvda Mevalonate pathway Zebrafish Epidermis Angiogenesis Abstract Background: The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoin- flammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the human MVD gene, in developing zebrafish. Methods: Morpholino antisense oligonucleotide technique was used to generate mvda loss-of-function phenotypes. Knockdown of mvda was confirmed by RT-PCR and Sanger sequencing. Scanning and transmission electron microscopy were performed to analyze the morphology of the epidermis. Angiogenesis study was presented using the Tg(fli1a:EGFP)yl transgenic strain. In addition, acridine orange staining was used to examine the apoptotic cells in vivo. Results: As expected, the mvda morphants showed abnormal morphology of the epidermis. Moreover, we observed ectopic sprouts in trunk angiogenesis and impaired formation of the caudal vein plexus in the mvda-deficient zebrafish. Besides, increased apoptosis was found throughout the tail, heart, and eyes in mvda zebrafish morphants. Conclusions: These findings indicated the essential role of mvda in the early development of zebrafish. This was the first in vivo knockdown study of the zebrafish mvda gene, which might offer insight into the biological function of the human MVD gene.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100217en10.1186/s40659-021-00341-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Mvda Mevalonate pathway Zebrafish Epidermis Angiogenesis
spellingShingle	Mvda Mevalonate pathway Zebrafish Epidermis Angiogenesis Wong,Wenghong Huang,Yike Wu,Zhuanbin Kong,Yu Luan,Jing Zhang,Qiaoan Pan,Jiewen Yan,Kexiang Zhang,Zhenghua Mvda is required for zebrafish early development
description	Abstract Background: The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoin- flammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the human MVD gene, in developing zebrafish. Methods: Morpholino antisense oligonucleotide technique was used to generate mvda loss-of-function phenotypes. Knockdown of mvda was confirmed by RT-PCR and Sanger sequencing. Scanning and transmission electron microscopy were performed to analyze the morphology of the epidermis. Angiogenesis study was presented using the Tg(fli1a:EGFP)yl transgenic strain. In addition, acridine orange staining was used to examine the apoptotic cells in vivo. Results: As expected, the mvda morphants showed abnormal morphology of the epidermis. Moreover, we observed ectopic sprouts in trunk angiogenesis and impaired formation of the caudal vein plexus in the mvda-deficient zebrafish. Besides, increased apoptosis was found throughout the tail, heart, and eyes in mvda zebrafish morphants. Conclusions: These findings indicated the essential role of mvda in the early development of zebrafish. This was the first in vivo knockdown study of the zebrafish mvda gene, which might offer insight into the biological function of the human MVD gene.
author	Wong,Wenghong Huang,Yike Wu,Zhuanbin Kong,Yu Luan,Jing Zhang,Qiaoan Pan,Jiewen Yan,Kexiang Zhang,Zhenghua
author_facet	Wong,Wenghong Huang,Yike Wu,Zhuanbin Kong,Yu Luan,Jing Zhang,Qiaoan Pan,Jiewen Yan,Kexiang Zhang,Zhenghua
author_sort	Wong,Wenghong
title	Mvda is required for zebrafish early development
title_short	Mvda is required for zebrafish early development
title_full	Mvda is required for zebrafish early development
title_fullStr	Mvda is required for zebrafish early development
title_full_unstemmed	Mvda is required for zebrafish early development
title_sort	mvda is required for zebrafish early development
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100217
work_keys_str_mv	AT wongwenghong mvdaisrequiredforzebrafishearlydevelopment AT huangyike mvdaisrequiredforzebrafishearlydevelopment AT wuzhuanbin mvdaisrequiredforzebrafishearlydevelopment AT kongyu mvdaisrequiredforzebrafishearlydevelopment AT luanjing mvdaisrequiredforzebrafishearlydevelopment AT zhangqiaoan mvdaisrequiredforzebrafishearlydevelopment AT panjiewen mvdaisrequiredforzebrafishearlydevelopment AT yankexiang mvdaisrequiredforzebrafishearlydevelopment AT zhangzhenghua mvdaisrequiredforzebrafishearlydevelopment
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Mvda is required for zebrafish early development

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