Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease

Abstract Background: Chagas disease is considered important and presents intense inflammatory and fibrotic processes induced by the perpetuation of the parasite in the affected tissues and organs. Therefore, it is necessary to inquire about the host defense and attack mechanisms to have a more deta...

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Autores principales:	Ballinas-Verdugo,Martha Alicia, Jiménez-Ortega,Rogelio Frank, Martínez-Martínez,Eduardo, Rivas,Nancy, Contreras-López,Erick Abraham, Carbó,Roxana, Sánchez,Fausto, Bojalil,Rafael, Márquez-Velasco,Ricardo, Sánchez-Muñoz,Fausto, Alejandre-Aguilar,Ricardo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	Trypanosoma cruzi Chagas disease microRNAs Extracellular vesicles (EVs) Chronic Chagas cardiomyopathy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100219
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spelling	oai:scielo:S0716-976020210001002192021-08-16Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas diseaseBallinas-Verdugo,Martha AliciaJiménez-Ortega,Rogelio FrankMartínez-Martínez,EduardoRivas,NancyContreras-López,Erick AbrahamCarbó,RoxanaSánchez,FaustoBojalil,RafaelMárquez-Velasco,RicardoSánchez-Muñoz,FaustoAlejandre-Aguilar,Ricardo Trypanosoma cruzi Chagas disease microRNAs Extracellular vesicles (EVs) Chronic Chagas cardiomyopathy Abstract Background: Chagas disease is considered important and presents intense inflammatory and fibrotic processes induced by the perpetuation of the parasite in the affected tissues and organs. Therefore, it is necessary to inquire about the host defense and attack mechanisms to have a more detailed knowledge about Chagas disease. Micro-RNAs are found in blood, tissues and extracellular vesicles. These small regulators of gene expression are involved in physiological and pathological processes in both mammals and parasites. Several microRNAs have deregulated expression in chagasic heart disease, although little is known about their extracellular expression. Our main objective was to evaluate the involvement of miR-21, miR-146a and miR-155 in several samples from mice infected with the TcI Ninoa strain from the acute and indeterminate phases. We also explored a potential functional association of the selected microRNAs using STRING software. This software identified 23 pathways associated with Trypanosoma cruzi infection. In addition, eleven genes were identified through bioinformatics analysis, and we found that SMAD family member 5 was downregulated in both phases. This gene serves as a mediator in the TGF-β signaling pathway. Thus, forty female mice of the CD1 strain were distributed into 4 groups and the expression levels of miR-21, miR-146a and miR-155 were measured in samples of heart tissue, total plasma and plasma extracellular vesicles by quantitative real-time polymerase chain reaction. Results: Overexpression of miR-21, miR-146a and miR-155 was observed in heart and plasma in both phases. Moreover, in extracellular vesicles miR-21 and miR-146a were also overexpressed in the acute phase, whereas in the indeterminate chronic phase we found only miR-146a up-regulated. Conclusions: The expression of inflammatory microRNAs miR-21, miR-146a and miR-155 were up-regulated in each of the samples from acutely and chronically infected mice. The relevant finding was that miR-146a was up-regulated in each sample in both phases; therefore, this miRNA could be a possible candidate biomarker in Chagas disease.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100219en10.1186/s40659-021-00345-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Trypanosoma cruzi Chagas disease microRNAs Extracellular vesicles (EVs) Chronic Chagas cardiomyopathy
spellingShingle	Trypanosoma cruzi Chagas disease microRNAs Extracellular vesicles (EVs) Chronic Chagas cardiomyopathy Ballinas-Verdugo,Martha Alicia Jiménez-Ortega,Rogelio Frank Martínez-Martínez,Eduardo Rivas,Nancy Contreras-López,Erick Abraham Carbó,Roxana Sánchez,Fausto Bojalil,Rafael Márquez-Velasco,Ricardo Sánchez-Muñoz,Fausto Alejandre-Aguilar,Ricardo Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease
description	Abstract Background: Chagas disease is considered important and presents intense inflammatory and fibrotic processes induced by the perpetuation of the parasite in the affected tissues and organs. Therefore, it is necessary to inquire about the host defense and attack mechanisms to have a more detailed knowledge about Chagas disease. Micro-RNAs are found in blood, tissues and extracellular vesicles. These small regulators of gene expression are involved in physiological and pathological processes in both mammals and parasites. Several microRNAs have deregulated expression in chagasic heart disease, although little is known about their extracellular expression. Our main objective was to evaluate the involvement of miR-21, miR-146a and miR-155 in several samples from mice infected with the TcI Ninoa strain from the acute and indeterminate phases. We also explored a potential functional association of the selected microRNAs using STRING software. This software identified 23 pathways associated with Trypanosoma cruzi infection. In addition, eleven genes were identified through bioinformatics analysis, and we found that SMAD family member 5 was downregulated in both phases. This gene serves as a mediator in the TGF-β signaling pathway. Thus, forty female mice of the CD1 strain were distributed into 4 groups and the expression levels of miR-21, miR-146a and miR-155 were measured in samples of heart tissue, total plasma and plasma extracellular vesicles by quantitative real-time polymerase chain reaction. Results: Overexpression of miR-21, miR-146a and miR-155 was observed in heart and plasma in both phases. Moreover, in extracellular vesicles miR-21 and miR-146a were also overexpressed in the acute phase, whereas in the indeterminate chronic phase we found only miR-146a up-regulated. Conclusions: The expression of inflammatory microRNAs miR-21, miR-146a and miR-155 were up-regulated in each of the samples from acutely and chronically infected mice. The relevant finding was that miR-146a was up-regulated in each sample in both phases; therefore, this miRNA could be a possible candidate biomarker in Chagas disease.
author	Ballinas-Verdugo,Martha Alicia Jiménez-Ortega,Rogelio Frank Martínez-Martínez,Eduardo Rivas,Nancy Contreras-López,Erick Abraham Carbó,Roxana Sánchez,Fausto Bojalil,Rafael Márquez-Velasco,Ricardo Sánchez-Muñoz,Fausto Alejandre-Aguilar,Ricardo
author_facet	Ballinas-Verdugo,Martha Alicia Jiménez-Ortega,Rogelio Frank Martínez-Martínez,Eduardo Rivas,Nancy Contreras-López,Erick Abraham Carbó,Roxana Sánchez,Fausto Bojalil,Rafael Márquez-Velasco,Ricardo Sánchez-Muñoz,Fausto Alejandre-Aguilar,Ricardo
author_sort	Ballinas-Verdugo,Martha Alicia
title	Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease
title_short	Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease
title_full	Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease
title_fullStr	Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease
title_full_unstemmed	Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease
title_sort	circulating mir-146a as a possible candidate biomarker in the indeterminate phase of chagas disease
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100219
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Circulating miR-146a as a possible candidate biomarker in the indeterminate phase of Chagas disease

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