miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia

Abstract Objective: This study aims to identify the effect of miR-146a-5p on trophoblast cell invasion as well as the mechanism in preeclampsia (PE). Methods: Expression levels of miR-146a-5p and Wnt2 in preeclamptic and normal placentae were quantified. Trophoblast cells (HTR-8) were separately t...

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Autores principales: Peng,Pingping, Song,Huamei, Xie,Chenghong, Zheng,Wenfei, Ma,Huigai, Xin,Dandan, Zhan,Jingqiong, Yuan,Xiaoqing, Chen,Aihua, Tao,Jing, Qin,Jufang
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2021
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100224
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spelling oai:scielo:S0716-976020210001002242021-10-14miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsiaPeng,PingpingSong,HuameiXie,ChenghongZheng,WenfeiMa,HuigaiXin,DandanZhan,JingqiongYuan,XiaoqingChen,AihuaTao,JingQin,Jufang Trophoblast cells miR-146a-5p Wnt2 Proliferation Migration Epithelial-mesenchymal transition Abstract Objective: This study aims to identify the effect of miR-146a-5p on trophoblast cell invasion as well as the mechanism in preeclampsia (PE). Methods: Expression levels of miR-146a-5p and Wnt2 in preeclamptic and normal placentae were quantified. Trophoblast cells (HTR-8) were separately transfected with miR-146a-5p mimic, miR-146a-5p inhibitor, pcDNA3.1-Wnt2 or sh-Wnt2, and then the expression levels of miR-146a-5p, Wnt2, and epithelial-mesenchymal transition (EMT)-related proteins (Vimentin, N-cadherin and E-cadherin) were measured. Moreover, the proliferative, migratory and invasive capacities of trophoblast cells were detected, respectively. Dual luciferase reporter assay determined the binding of miR-146a-5p and Wnt2. Results: Compared with normal placental tissues, the placentae from PE patients showed higher miR-146a-5p expression and lower Wnt2 expression. Transfection of miR-146a-5p inhibitor or pcDNA3.1-Wnt2 exerted pro-migratory and pro-invasive effects on HTR-8 cells and encouraged EMT in HTR-8 cells; transfection with miR-146a-5p mimic or sh-Wnt2 weakened the proliferative, migratory and invasive capacities as well as reduced EMT process of HTR-8 cells. Moreover, Wnt2 overexpression could partially counteract the suppressive effects of miR-146a-5p overexpression on the progression and EMT of HTR-8 cells. Conclusion: miR-146a-5p mediates trophoblast cell proliferation and invasion through regulating Wnt2 expression.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100224en10.1186/s40659-021-00351-5
institution Scielo Chile
collection Scielo Chile
language English
topic Trophoblast cells
miR-146a-5p
Wnt2
Proliferation
Migration
Epithelial-mesenchymal transition
spellingShingle Trophoblast cells
miR-146a-5p
Wnt2
Proliferation
Migration
Epithelial-mesenchymal transition
Peng,Pingping
Song,Huamei
Xie,Chenghong
Zheng,Wenfei
Ma,Huigai
Xin,Dandan
Zhan,Jingqiong
Yuan,Xiaoqing
Chen,Aihua
Tao,Jing
Qin,Jufang
miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia
description Abstract Objective: This study aims to identify the effect of miR-146a-5p on trophoblast cell invasion as well as the mechanism in preeclampsia (PE). Methods: Expression levels of miR-146a-5p and Wnt2 in preeclamptic and normal placentae were quantified. Trophoblast cells (HTR-8) were separately transfected with miR-146a-5p mimic, miR-146a-5p inhibitor, pcDNA3.1-Wnt2 or sh-Wnt2, and then the expression levels of miR-146a-5p, Wnt2, and epithelial-mesenchymal transition (EMT)-related proteins (Vimentin, N-cadherin and E-cadherin) were measured. Moreover, the proliferative, migratory and invasive capacities of trophoblast cells were detected, respectively. Dual luciferase reporter assay determined the binding of miR-146a-5p and Wnt2. Results: Compared with normal placental tissues, the placentae from PE patients showed higher miR-146a-5p expression and lower Wnt2 expression. Transfection of miR-146a-5p inhibitor or pcDNA3.1-Wnt2 exerted pro-migratory and pro-invasive effects on HTR-8 cells and encouraged EMT in HTR-8 cells; transfection with miR-146a-5p mimic or sh-Wnt2 weakened the proliferative, migratory and invasive capacities as well as reduced EMT process of HTR-8 cells. Moreover, Wnt2 overexpression could partially counteract the suppressive effects of miR-146a-5p overexpression on the progression and EMT of HTR-8 cells. Conclusion: miR-146a-5p mediates trophoblast cell proliferation and invasion through regulating Wnt2 expression.
author Peng,Pingping
Song,Huamei
Xie,Chenghong
Zheng,Wenfei
Ma,Huigai
Xin,Dandan
Zhan,Jingqiong
Yuan,Xiaoqing
Chen,Aihua
Tao,Jing
Qin,Jufang
author_facet Peng,Pingping
Song,Huamei
Xie,Chenghong
Zheng,Wenfei
Ma,Huigai
Xin,Dandan
Zhan,Jingqiong
Yuan,Xiaoqing
Chen,Aihua
Tao,Jing
Qin,Jufang
author_sort Peng,Pingping
title miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia
title_short miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia
title_full miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia
title_fullStr miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia
title_full_unstemmed miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia
title_sort mir-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia
publisher Sociedad de Biología de Chile
publishDate 2021
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100224
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AT taojing mir146a5pmediatedsuppressionontrophoblastcellprogressionandepithelialmesenchymaltransitioninpreeclampsia
AT qinjufang mir146a5pmediatedsuppressionontrophoblastcellprogressionandepithelialmesenchymaltransitioninpreeclampsia
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