Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training

Abstract Background: Heart failure (HF) is the leading cause of death in western countries. Cardiac dysfunction is accompanied by skeletal alterations resulting in muscle weakness and fatigue. Exercise is an accepted interventional approach correcting cardiac and skeletal dysfunction, thereby impro...

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Autores principales:	Böttner,Julia, Werner,Sarah, Adams,Volker, Knauth,Sarah, Kricke,Angela, Thiele,Holger, Büttner,Petra
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	Heart failure Transverse aortic constriction Moderate exercise Intervention Treadmill Peripheral cachexia Force production
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100225
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spelling	oai:scielo:S0716-976020210001002252021-10-14Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise trainingBöttner,JuliaWerner,SarahAdams,VolkerKnauth,SarahKricke,AngelaThiele,HolgerBüttner,Petra Heart failure Transverse aortic constriction Moderate exercise Intervention Treadmill Peripheral cachexia Force production Abstract Background: Heart failure (HF) is the leading cause of death in western countries. Cardiac dysfunction is accompanied by skeletal alterations resulting in muscle weakness and fatigue. Exercise is an accepted interventional approach correcting cardiac and skeletal dysfunction, thereby improving mortality, re-hospitalization and quality of life. Animal models are used to characterize underpinning mechanisms. Transverse aortic constriction (TAC) results in cardiac pressure overload and finally HF. Whether exercise training improves cardiac remodeling and peripheral cachexia in the TAC mouse model was not analyzed yet. In this study, 2 weeks post TAC animals were randomized into two groups either performing a moderate exercise program (five times per week at 60% VO2 max for 40 min for a total of 8 weeks) or staying sedentary. Results: In both TAC groups HF characteristics reduced ejection fraction (- 15% compared to sham, p < 0.001), cardiac remodeling (+ 22.5% cardiomyocyte cross sectional area compared to sham; p < 0.001) and coronary artery congestion (+ 34% diameter compared to sham; p = 0.008) were observed. Unexpectedly, peripheral cachexia was not detected. Furthermore, compared to sedentary group animals from the exercise group showed aggravated HF symptoms [heart area + 9% (p = 0.026), heart circumference + 7% (p = 0.002), right ventricular wall thickness - 30% (p = 0.003)] while muscle parameters were unchanged [Musculus soleus fiber diameter (p = 0.55), Musculus extensor digitorum longus contraction force (p = 0.90)]. Conclusion: The severe TAC model is inappropriate to study moderate exercise effects in HF with respect to cardiac and skeletal muscle improvements. Further, the phenotype induced by different TAC procedures should be well documented and taken into account when planning experiments.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100225en10.1186/s40659-021-00354-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Heart failure Transverse aortic constriction Moderate exercise Intervention Treadmill Peripheral cachexia Force production
spellingShingle	Heart failure Transverse aortic constriction Moderate exercise Intervention Treadmill Peripheral cachexia Force production Böttner,Julia Werner,Sarah Adams,Volker Knauth,Sarah Kricke,Angela Thiele,Holger Büttner,Petra Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training
description	Abstract Background: Heart failure (HF) is the leading cause of death in western countries. Cardiac dysfunction is accompanied by skeletal alterations resulting in muscle weakness and fatigue. Exercise is an accepted interventional approach correcting cardiac and skeletal dysfunction, thereby improving mortality, re-hospitalization and quality of life. Animal models are used to characterize underpinning mechanisms. Transverse aortic constriction (TAC) results in cardiac pressure overload and finally HF. Whether exercise training improves cardiac remodeling and peripheral cachexia in the TAC mouse model was not analyzed yet. In this study, 2 weeks post TAC animals were randomized into two groups either performing a moderate exercise program (five times per week at 60% VO2 max for 40 min for a total of 8 weeks) or staying sedentary. Results: In both TAC groups HF characteristics reduced ejection fraction (- 15% compared to sham, p < 0.001), cardiac remodeling (+ 22.5% cardiomyocyte cross sectional area compared to sham; p < 0.001) and coronary artery congestion (+ 34% diameter compared to sham; p = 0.008) were observed. Unexpectedly, peripheral cachexia was not detected. Furthermore, compared to sedentary group animals from the exercise group showed aggravated HF symptoms [heart area + 9% (p = 0.026), heart circumference + 7% (p = 0.002), right ventricular wall thickness - 30% (p = 0.003)] while muscle parameters were unchanged [Musculus soleus fiber diameter (p = 0.55), Musculus extensor digitorum longus contraction force (p = 0.90)]. Conclusion: The severe TAC model is inappropriate to study moderate exercise effects in HF with respect to cardiac and skeletal muscle improvements. Further, the phenotype induced by different TAC procedures should be well documented and taken into account when planning experiments.
author	Böttner,Julia Werner,Sarah Adams,Volker Knauth,Sarah Kricke,Angela Thiele,Holger Büttner,Petra
author_facet	Böttner,Julia Werner,Sarah Adams,Volker Knauth,Sarah Kricke,Angela Thiele,Holger Büttner,Petra
author_sort	Böttner,Julia
title	Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training
title_short	Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training
title_full	Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training
title_fullStr	Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training
title_full_unstemmed	Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training
title_sort	structural and functional alterations in heart and skeletal muscle following severe tac in mice: impact of moderate exercise training
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100225
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Structural and functional alterations in heart and skeletal muscle following severe TAC in mice: impact of moderate exercise training

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