QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM

During the embryonic development the heart is greatly dependent on the myocyte proliferation, which continues until the neonate. The myocardium regeneration during this period varies directly with the potential of cell proliferation. It is still debatable the proliferative and hypertrophic capacitie...

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Autores principales:	Xavier-Vidal,Ricardo, Cunha,Rosely C., Madi,Kalil
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 1997
Materias:	Heart development Fetus Myocyte Stereology Electron Microscopy Rat Development
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-98681997000200015
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spelling	oai:scielo:S0716-986819970002000152001-11-06QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUMXavier-Vidal,RicardoCunha,Rosely C.Madi,Kalil Heart development Fetus Myocyte Stereology Electron Microscopy Rat Development During the embryonic development the heart is greatly dependent on the myocyte proliferation, which continues until the neonate. The myocardium regeneration during this period varies directly with the potential of cell proliferation. It is still debatable the proliferative and hypertrophic capacities of the myocardium from embryonic to perinatal period. Five non-consanguineous rat fetuses at 18.5 and 20.5 days of gestation were utilized and fragments of the left ventricular wall were obtained, fixed and processed for routine electron microscopy. Stereological estimation made in semithin sections were obtained using 10 random microscopical fields in each heart with M-42 Test-System mounted in a Nikon CFW eyepiece (10X) and with immersion objective (100X). Qualitative electron microscopic study was performed. The results show the means of volumetric density values: a) Myocytes (including nuclei) = 69.3%; b) The Myocyte Nuclei alone = 6.5%; c) Connective Tissue (including vessels) = 30.7%. The Myocyte Nuclei has a mean of surface density equal to 0.073 µm²/µm³. The mean of numerical density of nuclei was obtained utilizing Disector's Method and the value found was 2357160 /mm³. The diameter of myocyte nuclei, considering like a sphere was 3.7 µm³. The EM presented myocytes well fixed with well-developed sarcomers disposed in an irregular form in the myocyte cytoplasm. The cardiac interstitium showed fibroblasts with characteristics of a great proteic synthesis. The volume of cardiomyocyte nuclei is smaller than that found in the humam myocardium during this period. There are differences in the numerical density of nuclei comparing rat and human. This article suggest binucleate cardiomyocyte during fetal period in rat as greater than was found in previous worksinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaRevista chilena de anatomía v.15 n.2 19971997-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-98681997000200015en10.4067/S0716-98681997000200015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Heart development Fetus Myocyte Stereology Electron Microscopy Rat Development
spellingShingle	Heart development Fetus Myocyte Stereology Electron Microscopy Rat Development Xavier-Vidal,Ricardo Cunha,Rosely C. Madi,Kalil QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM
description	During the embryonic development the heart is greatly dependent on the myocyte proliferation, which continues until the neonate. The myocardium regeneration during this period varies directly with the potential of cell proliferation. It is still debatable the proliferative and hypertrophic capacities of the myocardium from embryonic to perinatal period. Five non-consanguineous rat fetuses at 18.5 and 20.5 days of gestation were utilized and fragments of the left ventricular wall were obtained, fixed and processed for routine electron microscopy. Stereological estimation made in semithin sections were obtained using 10 random microscopical fields in each heart with M-42 Test-System mounted in a Nikon CFW eyepiece (10X) and with immersion objective (100X). Qualitative electron microscopic study was performed. The results show the means of volumetric density values: a) Myocytes (including nuclei) = 69.3%; b) The Myocyte Nuclei alone = 6.5%; c) Connective Tissue (including vessels) = 30.7%. The Myocyte Nuclei has a mean of surface density equal to 0.073 µm²/µm³. The mean of numerical density of nuclei was obtained utilizing Disector's Method and the value found was 2357160 /mm³. The diameter of myocyte nuclei, considering like a sphere was 3.7 µm³. The EM presented myocytes well fixed with well-developed sarcomers disposed in an irregular form in the myocyte cytoplasm. The cardiac interstitium showed fibroblasts with characteristics of a great proteic synthesis. The volume of cardiomyocyte nuclei is smaller than that found in the humam myocardium during this period. There are differences in the numerical density of nuclei comparing rat and human. This article suggest binucleate cardiomyocyte during fetal period in rat as greater than was found in previous works
author	Xavier-Vidal,Ricardo Cunha,Rosely C. Madi,Kalil
author_facet	Xavier-Vidal,Ricardo Cunha,Rosely C. Madi,Kalil
author_sort	Xavier-Vidal,Ricardo
title	QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM
title_short	QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM
title_full	QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM
title_fullStr	QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM
title_full_unstemmed	QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM
title_sort	quantitative study using semithin section of the rat fetal myocardium
publisher	Sociedad Chilena de Anatomía
publishDate	1997
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-98681997000200015
work_keys_str_mv	AT xaviervidalricardo quantitativestudyusingsemithinsectionoftheratfetalmyocardium AT cunharoselyc quantitativestudyusingsemithinsectionoftheratfetalmyocardium AT madikalil quantitativestudyusingsemithinsectionoftheratfetalmyocardium
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QUANTITATIVE STUDY USING SEMITHIN SECTION OF THE RAT FETAL MYOCARDIUM

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