FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS

Swine skeletal muscle fibers have traditionally been classified as types I, IIA, IIB and their hybrid forms by determining the activity of myosin ATPase (mATPase). More recently, molecular, electrophoretic and immunohistochemical approaches have allowed the identification of IIx isoform-containing f...

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Autores principales:	Graziotti,Guillermo H., Delhon,Gustavo, Ríos,Clara M., Rivero,José-Luis L.
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2001
Materias:	Pig Muscular fibers Myosin Isoforms Histochemical Immunohistochemical
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-98682001000200008
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spelling	oai:scielo:S0716-986820010002000082001-11-30FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODSGraziotti,Guillermo H.Delhon,GustavoRíos,Clara M.Rivero,José-Luis L. Pig Muscular fibers Myosin Isoforms Histochemical Immunohistochemical Swine skeletal muscle fibers have traditionally been classified as types I, IIA, IIB and their hybrid forms by determining the activity of myosin ATPase (mATPase). More recently, molecular, electrophoretic and immunohistochemical approaches have allowed the identification of IIx isoform-containing fibers. In this study, myosin isoforms present in different types of muscle fibers were identified by combining histochemical and immunohistochemical methods. Samples from the M. longissimus dorsi were collected from primiparus pigs (mean body weight, 150 Kg) 45 minutes after slaughtering and subsequently frozen in dry ice-cooled acetone. Serial cross sections were processed for mATPase and NADH-TR activities and for immunohistochemistry. Five histochemical fiber groups were identified: type I (dark), type IIA (light) and three intermediate types. A panel of monoclonal antibodies identified the slow ßI, IIa, IIx and IIb isoforms present in types I, IIA, IIX and IIB fibers and the hybrid group IIAXinfo:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaRevista chilena de anatomía v.19 n.2 20012001-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-98682001000200008en10.4067/S0716-98682001000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pig Muscular fibers Myosin Isoforms Histochemical Immunohistochemical
spellingShingle	Pig Muscular fibers Myosin Isoforms Histochemical Immunohistochemical Graziotti,Guillermo H. Delhon,Gustavo Ríos,Clara M. Rivero,José-Luis L. FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS
description	Swine skeletal muscle fibers have traditionally been classified as types I, IIA, IIB and their hybrid forms by determining the activity of myosin ATPase (mATPase). More recently, molecular, electrophoretic and immunohistochemical approaches have allowed the identification of IIx isoform-containing fibers. In this study, myosin isoforms present in different types of muscle fibers were identified by combining histochemical and immunohistochemical methods. Samples from the M. longissimus dorsi were collected from primiparus pigs (mean body weight, 150 Kg) 45 minutes after slaughtering and subsequently frozen in dry ice-cooled acetone. Serial cross sections were processed for mATPase and NADH-TR activities and for immunohistochemistry. Five histochemical fiber groups were identified: type I (dark), type IIA (light) and three intermediate types. A panel of monoclonal antibodies identified the slow ßI, IIa, IIx and IIb isoforms present in types I, IIA, IIX and IIB fibers and the hybrid group IIAX
author	Graziotti,Guillermo H. Delhon,Gustavo Ríos,Clara M. Rivero,José-Luis L.
author_facet	Graziotti,Guillermo H. Delhon,Gustavo Ríos,Clara M. Rivero,José-Luis L.
author_sort	Graziotti,Guillermo H.
title	FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS
title_short	FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS
title_full	FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS
title_fullStr	FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS
title_full_unstemmed	FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS
title_sort	fiber muscle types in adult female pigs as determined by combinating histochemical and immunohistochemical methods
publisher	Sociedad Chilena de Anatomía
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-98682001000200008
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FIBER MUSCLE TYPES IN ADULT FEMALE PIGS AS DETERMINED BY COMBINATING HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL METHODS

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