Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.

Rapid, large-scale genome as well as diagnostic DNA sequencing projects are, at present, dependent on the use of sensitive automated sequencers that rely on the detection of fluorescent signals. This emission is not an intrinsic property of the biomolecules but is a property of an optical label that...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Igloi,Gabor L
Lenguaje:	Spanish / Castilian
Publicado:	Pontificia Universidad Católica de Valparaíso 1998
Materias:	Automated sequencing Non-radioactive labelling Nucleic acid analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34581998000100003
record_format	dspace
spelling	oai:scielo:S0717-345819980001000032005-12-15Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.Igloi,Gabor L Automated sequencing Non-radioactive labelling Nucleic acid analysis Rapid, large-scale genome as well as diagnostic DNA sequencing projects are, at present, dependent on the use of sensitive automated sequencers that rely on the detection of fluorescent signals. This emission is not an intrinsic property of the biomolecules but is a property of an optical label that must be incorporated before, during or after the sequence specific reaction. The choice of strategy, that is to be reviewed here, is a function of both the chemistry and the enzymology of the system as well as the nature of the fractionation and the physical parameters of the detection. One may differentiate beween the labelling of the primer, incorporation of the label during elongation or base specific termination with labelled terminators. In reviewing the development of each of these strategies, the conclusion is reached that, whereas labelled primers have universal applicability, the current generation of fluorescent terminators have in, conjunction with appropriate DNA polymerases, attained a refinement that strongly favours their use. However, since they are not available for all commercial sequencing systems, the alternative procedures are not merely of historic interest but are an essential component in DNA sequencing protocols. The possibility of automated direct sequence analysis of RNA has not been ignored completelyinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.1 n.1 19981998-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100003es10.4067/S0717-34581998000100003
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Automated sequencing Non-radioactive labelling Nucleic acid analysis
spellingShingle	Automated sequencing Non-radioactive labelling Nucleic acid analysis Igloi,Gabor L Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.
description	Rapid, large-scale genome as well as diagnostic DNA sequencing projects are, at present, dependent on the use of sensitive automated sequencers that rely on the detection of fluorescent signals. This emission is not an intrinsic property of the biomolecules but is a property of an optical label that must be incorporated before, during or after the sequence specific reaction. The choice of strategy, that is to be reviewed here, is a function of both the chemistry and the enzymology of the system as well as the nature of the fractionation and the physical parameters of the detection. One may differentiate beween the labelling of the primer, incorporation of the label during elongation or base specific termination with labelled terminators. In reviewing the development of each of these strategies, the conclusion is reached that, whereas labelled primers have universal applicability, the current generation of fluorescent terminators have in, conjunction with appropriate DNA polymerases, attained a refinement that strongly favours their use. However, since they are not available for all commercial sequencing systems, the alternative procedures are not merely of historic interest but are an essential component in DNA sequencing protocols. The possibility of automated direct sequence analysis of RNA has not been ignored completely
author	Igloi,Gabor L
author_facet	Igloi,Gabor L
author_sort	Igloi,Gabor L
title	Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.
title_short	Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.
title_full	Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.
title_fullStr	Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.
title_full_unstemmed	Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.
title_sort	strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	1998
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100003
work_keys_str_mv	AT igloigaborl strategiesforintroducingnonradioactivelabelsduringtheautomatedsequenceanalysisofnucleicacids
_version_	1718441677954220032

Strategies for introducing non-radioactive labels during the automated sequence analysis of nucleic acids.

Ejemplares similares