RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.

RNA editing in plant mitochondria is a post-transcriptional process involving the partial change of C residues into U. These C to U changes lead to the synthesis of proteins with an amino acid sequence different to that predicted from the gene. Proteins produced from edited mRNAs are more similar to...

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Autores principales:	Araya,Alexandre, Zabaleta,Eduardo, Blanc,Valérie, Bégu,Dominique, Hernould,Michel, Mouras,Armand, Litvak,Simon
Lenguaje:	Spanish / Castilian
Publicado:	Pontificia Universidad Católica de Valparaíso 1998
Materias:	Plant mitochondria RNA editing cytidine deamination Cytoplasmic male sterility (CMS Fertility restoration
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100004
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spelling	oai:scielo:S0717-345819980001000042005-12-15RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.Araya,AlexandreZabaleta,EduardoBlanc,ValérieBégu,DominiqueHernould,MichelMouras,ArmandLitvak,Simon Plant mitochondria RNA editing cytidine deamination Cytoplasmic male sterility (CMS Fertility restoration RNA editing in plant mitochondria is a post-transcriptional process involving the partial change of C residues into U. These C to U changes lead to the synthesis of proteins with an amino acid sequence different to that predicted from the gene. Proteins produced from edited mRNAs are more similar to those from organisms where this process is absent. This biochemical process involves cytidine deamination. The cytoplasmic male sterility (CMS) phenotype generated by the incompatibility between the nuclear and the mitochondrial genomes is an important agronomical trait which prevents inbreeding and favors hybrid production. The hypothesis that RNA editing leads to functional proteins has been proposed. This hypothesis was tested by constructing transgenic plants expressing a mitochondrial protein translated from unedited mRNA. The transgenic "unedited" protein was addressed to the mitochondria leading to the appearance of mitochondrial dysfunction and generating the male sterile phenotype in transgenic tobacco plants. Male sterile plants were also obtained by expressing specifically a bacterial ribonuclease in the anthers. The economical benefits of artificially engineered male-sterile plants or carrying the (native) spontaneous CMS phenotype, implies the restoration to obtain fertile hybrids that will be used in agriculture. Restoration to fertility of transgenic plants was obtained either by crossing male-sterile plants carrying the "unedited" mRNA with plants carrying the same RNA, but in the antisense orientation or, in the case of plants expresing the ribonuclease, by crossing male-sterile plants with plants expressing an inhibitor specific of this enzymeinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.1 n.1 19981998-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100004es10.4067/S0717-34581998000100004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Plant mitochondria RNA editing cytidine deamination Cytoplasmic male sterility (CMS Fertility restoration
spellingShingle	Plant mitochondria RNA editing cytidine deamination Cytoplasmic male sterility (CMS Fertility restoration Araya,Alexandre Zabaleta,Eduardo Blanc,Valérie Bégu,Dominique Hernould,Michel Mouras,Armand Litvak,Simon RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
description	RNA editing in plant mitochondria is a post-transcriptional process involving the partial change of C residues into U. These C to U changes lead to the synthesis of proteins with an amino acid sequence different to that predicted from the gene. Proteins produced from edited mRNAs are more similar to those from organisms where this process is absent. This biochemical process involves cytidine deamination. The cytoplasmic male sterility (CMS) phenotype generated by the incompatibility between the nuclear and the mitochondrial genomes is an important agronomical trait which prevents inbreeding and favors hybrid production. The hypothesis that RNA editing leads to functional proteins has been proposed. This hypothesis was tested by constructing transgenic plants expressing a mitochondrial protein translated from unedited mRNA. The transgenic "unedited" protein was addressed to the mitochondria leading to the appearance of mitochondrial dysfunction and generating the male sterile phenotype in transgenic tobacco plants. Male sterile plants were also obtained by expressing specifically a bacterial ribonuclease in the anthers. The economical benefits of artificially engineered male-sterile plants or carrying the (native) spontaneous CMS phenotype, implies the restoration to obtain fertile hybrids that will be used in agriculture. Restoration to fertility of transgenic plants was obtained either by crossing male-sterile plants carrying the "unedited" mRNA with plants carrying the same RNA, but in the antisense orientation or, in the case of plants expresing the ribonuclease, by crossing male-sterile plants with plants expressing an inhibitor specific of this enzyme
author	Araya,Alexandre Zabaleta,Eduardo Blanc,Valérie Bégu,Dominique Hernould,Michel Mouras,Armand Litvak,Simon
author_facet	Araya,Alexandre Zabaleta,Eduardo Blanc,Valérie Bégu,Dominique Hernould,Michel Mouras,Armand Litvak,Simon
author_sort	Araya,Alexandre
title	RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
title_short	RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
title_full	RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
title_fullStr	RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
title_full_unstemmed	RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
title_sort	rna editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	1998
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100004
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RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.

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