Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidases

Ceriporiopsis subvermispora is a white-rot basidiomycete that produces several isoenzymes of manganese peroxidase (MnP· ). A cDNA of one of them (MnP13-1) has been isolated and sequenced. The deduced aminoacid sequence shows about 60% similarity with the MnPs from Phanerochaete chrysosporium. Based...

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Autores principales: Canales,Mauricio, Lobos,Sergio, Vicuña,Rafael
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 1998
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000200006
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spelling oai:scielo:S0717-345819980002000062003-08-18Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidasesCanales,MauricioLobos,SergioVicuña,Rafael Ceriporiopsis subvermispora is a white-rot basidiomycete that produces several isoenzymes of manganese peroxidase (MnP· ). A cDNA of one of them (MnP13-1) has been isolated and sequenced. The deduced aminoacid sequence shows about 60% similarity with the MnPs from Phanerochaete chrysosporium. Based on the crystal structures of MnP and lignin peroxidase (LiP) from P. chrysosporium, and of a peroxidase from Arthromyces ramosus (ARP), we have modeled by homology the three dimensional structure of MnP13-1 using standard modeling procedures. Local molecular mechanics optimization performed in the region corresponding to the binding sites of Ca2+ and Mn2+ in MnP13-1 demonstrated that the stereochemistry and the geometry of binding are conserved in both MnPs. A putative aromatic binding site in MnP13-1 is described. We also report structural differences between the two MnPs, arising from the insertion in MnP13-1 of the sequences TGGN between residues S230 and D231 and TDSP at the C-terminal, both of which may have functional significance.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.1 n.2 19981998-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000200006en10.4067/S0717-34581998000200006
institution Scielo Chile
collection Scielo Chile
language English
description Ceriporiopsis subvermispora is a white-rot basidiomycete that produces several isoenzymes of manganese peroxidase (MnP· ). A cDNA of one of them (MnP13-1) has been isolated and sequenced. The deduced aminoacid sequence shows about 60% similarity with the MnPs from Phanerochaete chrysosporium. Based on the crystal structures of MnP and lignin peroxidase (LiP) from P. chrysosporium, and of a peroxidase from Arthromyces ramosus (ARP), we have modeled by homology the three dimensional structure of MnP13-1 using standard modeling procedures. Local molecular mechanics optimization performed in the region corresponding to the binding sites of Ca2+ and Mn2+ in MnP13-1 demonstrated that the stereochemistry and the geometry of binding are conserved in both MnPs. A putative aromatic binding site in MnP13-1 is described. We also report structural differences between the two MnPs, arising from the insertion in MnP13-1 of the sequences TGGN between residues S230 and D231 and TDSP at the C-terminal, both of which may have functional significance.
author Canales,Mauricio
Lobos,Sergio
Vicuña,Rafael
spellingShingle Canales,Mauricio
Lobos,Sergio
Vicuña,Rafael
Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidases
author_facet Canales,Mauricio
Lobos,Sergio
Vicuña,Rafael
author_sort Canales,Mauricio
title Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidases
title_short Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidases
title_full Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidases
title_fullStr Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidases
title_full_unstemmed Molecular modeling of manganese peroxidase from the lignin-degrading fungus Ceriporiopsis subvermispora and structural comparison with other peroxidases
title_sort molecular modeling of manganese peroxidase from the lignin-degrading fungus ceriporiopsis subvermispora and structural comparison with other peroxidases
publisher Pontificia Universidad Católica de Valparaíso
publishDate 1998
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000200006
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AT lobossergio molecularmodelingofmanganeseperoxidasefromthelignindegradingfungusceriporiopsissubvermisporaandstructuralcomparisonwithotherperoxidases
AT vicunarafael molecularmodelingofmanganeseperoxidasefromthelignindegradingfungusceriporiopsissubvermisporaandstructuralcomparisonwithotherperoxidases
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