Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm

Yam edible tubers feed million of peoples in the intertropical area, where they represent 12% of human feeding. However, as a vegetatively propagated crop, yam is seriously affected by an accumulation of pathogens. Establishing in vitro germplasm collection is a process that cleans the plants from a...

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Autores principales:	Malaurie,Bernard, Trouslot,Marie-France, Berthaud,Julien, Bousalem,Mustapha, Pinel,Agnès, Dubern,Jean
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 1998
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300001
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spelling	oai:scielo:S0717-345819980003000012005-12-22Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasmMalaurie,BernardTrouslot,Marie-FranceBerthaud,JulienBousalem,MustaphaPinel,AgnèsDubern,Jean Yam edible tubers feed million of peoples in the intertropical area, where they represent 12% of human feeding. However, as a vegetatively propagated crop, yam is seriously affected by an accumulation of pathogens. Establishing in vitro germplasm collection is a process that cleans the plants from all diseases but viruses. It gives a good control on the preservation of the yam genetic resources and facilitates international exchanges of healthy plant material. Two kinds of in vitro germplasm preservation were considered : slow growth condition culture for mid-term preservation, and cryopreservation using the encapsulation/dehydration technique for long-term preservation. Virus eradication was approached by meristem culture and chemo and thermotherapy. Production of virus-free plants was controlled by ELISA. We succeeded in the introduction and maintenance of 20 yam species, under slow growth conditions. Cryopreservation was applied successfully on two edible yam species, Dioscorea. alata L and D. bulbifera L. Virus-free plants were obtained by meristem culture in D. cayenensis-D. rotundata complex and D. praehensilis. Indexation allowed the detection of different virus (poty-, potex-, badna- and cucumovirus), where the most important potyvirus was YMV. Mid-term conservation of yam germplasm is used routinely, and from these conditions a direct acclimatization is possible. On the cryopreservation aspect, experiments are under way to apply the optimized protocol to genotypes which are more representative of the diversity, to insure a routinely use. More work can be conducted now on virus eradication, based on knowledge accumulated on potyvirus diversity, on several tests available for yam indexing (ELISA, rt/PCR, monoclonal antibodies) and on new sanitation techniques.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.1 n.3 19981998-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300001en10.4067/S0717-34581998000300001
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Yam edible tubers feed million of peoples in the intertropical area, where they represent 12% of human feeding. However, as a vegetatively propagated crop, yam is seriously affected by an accumulation of pathogens. Establishing in vitro germplasm collection is a process that cleans the plants from all diseases but viruses. It gives a good control on the preservation of the yam genetic resources and facilitates international exchanges of healthy plant material. Two kinds of in vitro germplasm preservation were considered : slow growth condition culture for mid-term preservation, and cryopreservation using the encapsulation/dehydration technique for long-term preservation. Virus eradication was approached by meristem culture and chemo and thermotherapy. Production of virus-free plants was controlled by ELISA. We succeeded in the introduction and maintenance of 20 yam species, under slow growth conditions. Cryopreservation was applied successfully on two edible yam species, Dioscorea. alata L and D. bulbifera L. Virus-free plants were obtained by meristem culture in D. cayenensis-D. rotundata complex and D. praehensilis. Indexation allowed the detection of different virus (poty-, potex-, badna- and cucumovirus), where the most important potyvirus was YMV. Mid-term conservation of yam germplasm is used routinely, and from these conditions a direct acclimatization is possible. On the cryopreservation aspect, experiments are under way to apply the optimized protocol to genotypes which are more representative of the diversity, to insure a routinely use. More work can be conducted now on virus eradication, based on knowledge accumulated on potyvirus diversity, on several tests available for yam indexing (ELISA, rt/PCR, monoclonal antibodies) and on new sanitation techniques.
author	Malaurie,Bernard Trouslot,Marie-France Berthaud,Julien Bousalem,Mustapha Pinel,Agnès Dubern,Jean
spellingShingle	Malaurie,Bernard Trouslot,Marie-France Berthaud,Julien Bousalem,Mustapha Pinel,Agnès Dubern,Jean Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm
author_facet	Malaurie,Bernard Trouslot,Marie-France Berthaud,Julien Bousalem,Mustapha Pinel,Agnès Dubern,Jean
author_sort	Malaurie,Bernard
title	Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm
title_short	Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm
title_full	Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm
title_fullStr	Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm
title_full_unstemmed	Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm
title_sort	medium-term and long-term in vitro conservation and safe international exchange of yam (dioscorea spp.) germplasm
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	1998
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300001
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Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm

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