Cutinase structure, function and biocatalytic applications
This review analyses the role of cutinases in nature and their potential biotechnological applications. The cloning and expression of a fungal cutinase from Fusarium solani f. pisi, in Escherichia coli and Saccharomyces cerevisiae hosts are described. The three dimensional structure of this cutinase...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
1998
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300006 |
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oai:scielo:S0717-345819980003000062005-12-22Cutinase structure, function and biocatalytic applicationsCarvalho,Cristina M. L.Aires-Barros,Maria RaquelCabral,Joaquim M. S. This review analyses the role of cutinases in nature and their potential biotechnological applications. The cloning and expression of a fungal cutinase from Fusarium solani f. pisi, in Escherichia coli and Saccharomyces cerevisiae hosts are described. The three dimensional structure of this cutinase is also analysed and its function as a lipase discussed and compared with other lipases. The biocatalytic applications of cutinase are described taking into account the preparation of different cutinase forms and the media where the different types of enzymatic reactions have been performed, namely hydrolysis, esterification, transesterification and resolution of racemic mixtures. The stability of cutinase preparations is discussed, particularly in anionic reversed micelles considering the role of hexanol as substrate, co-surfactant and stabilizer. Process development based on the operation of cutinase reactors is also reviewed.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.1 n.3 19981998-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300006en10.4067/S0717-34581998000300006 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
This review analyses the role of cutinases in nature and their potential biotechnological applications. The cloning and expression of a fungal cutinase from Fusarium solani f. pisi, in Escherichia coli and Saccharomyces cerevisiae hosts are described. The three dimensional structure of this cutinase is also analysed and its function as a lipase discussed and compared with other lipases. The biocatalytic applications of cutinase are described taking into account the preparation of different cutinase forms and the media where the different types of enzymatic reactions have been performed, namely hydrolysis, esterification, transesterification and resolution of racemic mixtures. The stability of cutinase preparations is discussed, particularly in anionic reversed micelles considering the role of hexanol as substrate, co-surfactant and stabilizer. Process development based on the operation of cutinase reactors is also reviewed. |
| author |
Carvalho,Cristina M. L. Aires-Barros,Maria Raquel Cabral,Joaquim M. S. |
| spellingShingle |
Carvalho,Cristina M. L. Aires-Barros,Maria Raquel Cabral,Joaquim M. S. Cutinase structure, function and biocatalytic applications |
| author_facet |
Carvalho,Cristina M. L. Aires-Barros,Maria Raquel Cabral,Joaquim M. S. |
| author_sort |
Carvalho,Cristina M. L. |
| title |
Cutinase structure, function and biocatalytic applications |
| title_short |
Cutinase structure, function and biocatalytic applications |
| title_full |
Cutinase structure, function and biocatalytic applications |
| title_fullStr |
Cutinase structure, function and biocatalytic applications |
| title_full_unstemmed |
Cutinase structure, function and biocatalytic applications |
| title_sort |
cutinase structure, function and biocatalytic applications |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
1998 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300006 |
| work_keys_str_mv |
AT carvalhocristinaml cutinasestructurefunctionandbiocatalyticapplications AT airesbarrosmariaraquel cutinasestructurefunctionandbiocatalyticapplications AT cabraljoaquimms cutinasestructurefunctionandbiocatalyticapplications |
| _version_ |
1718441681962926080 |