In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection

Agrobacterium rhizogenes infection at the base of microcuttings in vitro can improve the rooting of some fruit species. A study was carried out comparing rooting of almond, apple, plum, Pyrus pyraster and two hybrid rootstocks, when infected with A. rhizogenes strain 1855, with and without the addit...

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Autores principales:	Damiano,Carmine, Monticelli,Simona
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 1998
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300008
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id	oai:scielo:S0717-34581998000300008
record_format	dspace
spelling	oai:scielo:S0717-345819980003000082005-12-22In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infectionDamiano,CarmineMonticelli,Simona Agrobacterium rhizogenes infection at the base of microcuttings in vitro can improve the rooting of some fruit species. A study was carried out comparing rooting of almond, apple, plum, Pyrus pyraster and two hybrid rootstocks, when infected with A. rhizogenes strain 1855, with and without the addition of hormones. Three responses occurred: genotypes rooted without auxins; genotypes rooted only with auxins; genotypes rooted only after infection. All genotypes rooted after bacterial infection. In the first group the auxins increased rooting percentages. No substantial differences were found with and without infection in hormone free media, while the rooting percentages tended to decrease with the combination auxin/infection. In the second group, infection on hormone free media increased rooting; in addition there was a synergistic effect between auxins and infection in pears. In the third group only infection induced rooting. A random sample of roots obtained from infection was molecularly analysed. Amplification of the sequences of rolB and vir genes was done using PCR. Roots non-transgenic and confirmed as transgenic were, respectively, 67 % and 6.8 % respectively. In the remaining 26.2% both genes appeared, thus it was not possible to confirm their transgenic nature. Some microcuttings showed both transformed and non-transformed roots.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.1 n.3 19981998-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300008en10.4067/S0717-34581998000300008
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Agrobacterium rhizogenes infection at the base of microcuttings in vitro can improve the rooting of some fruit species. A study was carried out comparing rooting of almond, apple, plum, Pyrus pyraster and two hybrid rootstocks, when infected with A. rhizogenes strain 1855, with and without the addition of hormones. Three responses occurred: genotypes rooted without auxins; genotypes rooted only with auxins; genotypes rooted only after infection. All genotypes rooted after bacterial infection. In the first group the auxins increased rooting percentages. No substantial differences were found with and without infection in hormone free media, while the rooting percentages tended to decrease with the combination auxin/infection. In the second group, infection on hormone free media increased rooting; in addition there was a synergistic effect between auxins and infection in pears. In the third group only infection induced rooting. A random sample of roots obtained from infection was molecularly analysed. Amplification of the sequences of rolB and vir genes was done using PCR. Roots non-transgenic and confirmed as transgenic were, respectively, 67 % and 6.8 % respectively. In the remaining 26.2% both genes appeared, thus it was not possible to confirm their transgenic nature. Some microcuttings showed both transformed and non-transformed roots.
author	Damiano,Carmine Monticelli,Simona
spellingShingle	Damiano,Carmine Monticelli,Simona In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection
author_facet	Damiano,Carmine Monticelli,Simona
author_sort	Damiano,Carmine
title	In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection
title_short	In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection
title_full	In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection
title_fullStr	In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection
title_full_unstemmed	In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection
title_sort	in vitro fruit trees rooting by agrobacterium rhizogenes wild type infection
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	1998
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000300008
work_keys_str_mv	AT damianocarmine invitrofruittreesrootingbyagrobacteriumrhizogeneswildtypeinfection AT monticellisimona invitrofruittreesrootingbyagrobacteriumrhizogeneswildtypeinfection
_version_	1718441682531254272

In vitro fruit trees rooting by Agrobacterium rhizogenes wild type infection

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