Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis

Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis

Citrus tristeza closterovirus (CTV), is a phloem-limited virus transmitted by aphids in a semipersistent manner. The genome of CTV is composed of a ssRNA with two capsid proteins: CP, covering about 95% of the particle length, and a diverged coat protein (dCP), present only in one end of the particl...

Descripción completa

Guardado en:
Detalles Bibliográficos
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 1999
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581999000100005
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0717-34581999000100005
record_format dspace
spelling oai:scielo:S0717-345819990001000052003-08-18Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis Citrus tristeza closterovirus (CTV), is a phloem-limited virus transmitted by aphids in a semipersistent manner. The genome of CTV is composed of a ssRNA with two capsid proteins: CP, covering about 95% of the particle length, and a diverged coat protein (dCP), present only in one end of the particle, forming a rattlesnake structure. dCP is the product of p27 gene for which it is also postulated a function in the transmissibility by aphid vectors. Hybridization analysis showed a p27 gene region, which exhibits different patterns with two probes derived from two biological distinct CTV isolates. In an attempt to screen whether that gene region differs in mild and severe strains, six CTV isolates belonging to different biogroups were compared for variations in their p27 gene by analysis of single-strand conformation polymorphism (SSCP). The p27 gene was reverse transcribed and amplified by PCR and thirty clones of each isolate were obtained. From each clone, two fragments of the gene were amplified by PCR: fragment (a), 459 bp long, and fragment (b), 281 bp long. Sequence variations in both gene fragments were studied by SSCP analysis. A variety of SSCP patterns was obtained from each isolate, being isolates belonging to the groups II-IV and III those with the higher and lower number of them. Moreover, SSCP analysis provided a rapid procedure to screen the genetic heterogeneity of the viral isolates reducing considerably the amount of nucleic acid sequenciation necessary to gain that knowledge.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.2 n.1 19991999-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581999000100005en10.4067/S0717-34581999000100005
institution Scielo Chile
collection Scielo Chile
language English
description Citrus tristeza closterovirus (CTV), is a phloem-limited virus transmitted by aphids in a semipersistent manner. The genome of CTV is composed of a ssRNA with two capsid proteins: CP, covering about 95% of the particle length, and a diverged coat protein (dCP), present only in one end of the particle, forming a rattlesnake structure. dCP is the product of p27 gene for which it is also postulated a function in the transmissibility by aphid vectors. Hybridization analysis showed a p27 gene region, which exhibits different patterns with two probes derived from two biological distinct CTV isolates. In an attempt to screen whether that gene region differs in mild and severe strains, six CTV isolates belonging to different biogroups were compared for variations in their p27 gene by analysis of single-strand conformation polymorphism (SSCP). The p27 gene was reverse transcribed and amplified by PCR and thirty clones of each isolate were obtained. From each clone, two fragments of the gene were amplified by PCR: fragment (a), 459 bp long, and fragment (b), 281 bp long. Sequence variations in both gene fragments were studied by SSCP analysis. A variety of SSCP patterns was obtained from each isolate, being isolates belonging to the groups II-IV and III those with the higher and lower number of them. Moreover, SSCP analysis provided a rapid procedure to screen the genetic heterogeneity of the viral isolates reducing considerably the amount of nucleic acid sequenciation necessary to gain that knowledge.
title Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis
spellingShingle Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis
title_short Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis
title_full Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis
title_fullStr Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis
title_full_unstemmed Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis
title_sort sequence variability in p27 gene of citrus tristeza virus (ctv) revealed by sscp analysis
publisher Pontificia Universidad Católica de Valparaíso
publishDate 1999
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581999000100005
_version_ 1718441684125089792

Ejemplares similares