Isolation by PCR-based methods of a plant antifungal polygalacturonase-inhibiting protein gene

A polygalacturonase-inhibiting protein (pgip) gene from Malus domestica cv Granny Smith apple fruit was cloned by degenerate oligo-primed polymerase chain reaction (PCR) and Inverse PCR. An alignment of the pear and bean PGIP sequences was used to design degenerate PCR primers in highly conserved re...

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Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 1999
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581999000300004
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