Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida

Hairy root cultures of Brugmansia candida (Solanaceae), a South American plant which produces scopolamine and hyoscyamine, were exposed to different elicitors (jasmonic acid (JA) and aluminum chloride (AlCl3)) in order to increase their productivity and/or stimulate their liberation. Hairy roots of...

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Autores principales:	Spollansky,Tatiana C., Pitta-Alvarez,Sandra I., Giulietti,Ana M.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2000
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000100006
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spelling	oai:scielo:S0717-345820000001000062003-08-18Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candidaSpollansky,Tatiana C.Pitta-Alvarez,Sandra I.Giulietti,Ana M. Hairy root cultures of Brugmansia candida (Solanaceae), a South American plant which produces scopolamine and hyoscyamine, were exposed to different elicitors (jasmonic acid (JA) and aluminum chloride (AlCl3)) in order to increase their productivity and/or stimulate their liberation. Hairy roots of 19-day old cultures (exponential phase) were exposed to these elicitors for 24 and 48 hours. The effects on alkaloid accumulation and release into the medium were evaluated. JA was tested at 2.5 and 25 m g/ml. After 24 hours, JA promoted the release of hyoscyamine (~1200%) when the highest concentration was used. Therefore, the positive effects seen with JA could possibly be attributed in part to ethanol (EtOH), the solvent in which the acid was dissolved. At the lowest concentration tested, JA promoted an increase on scopolamine accumulation (30%) after 48 hours of exposure. When exposed to AlCl3 for 48 hours and at concentrations of 25 and 250m M, scopolamine and hyoscyamine accumulation increased in the roots (43-83%). After 48 hours of treatment with the highest concentration of AlCl3, release of scopolamine into the medium increased approximately 150%. <A NAME="Article"></A>info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.3 n.1 20002000-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000100006en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Hairy root cultures of Brugmansia candida (Solanaceae), a South American plant which produces scopolamine and hyoscyamine, were exposed to different elicitors (jasmonic acid (JA) and aluminum chloride (AlCl3)) in order to increase their productivity and/or stimulate their liberation. Hairy roots of 19-day old cultures (exponential phase) were exposed to these elicitors for 24 and 48 hours. The effects on alkaloid accumulation and release into the medium were evaluated. JA was tested at 2.5 and 25 m g/ml. After 24 hours, JA promoted the release of hyoscyamine (~1200%) when the highest concentration was used. Therefore, the positive effects seen with JA could possibly be attributed in part to ethanol (EtOH), the solvent in which the acid was dissolved. At the lowest concentration tested, JA promoted an increase on scopolamine accumulation (30%) after 48 hours of exposure. When exposed to AlCl3 for 48 hours and at concentrations of 25 and 250m M, scopolamine and hyoscyamine accumulation increased in the roots (43-83%). After 48 hours of treatment with the highest concentration of AlCl3, release of scopolamine into the medium increased approximately 150%. <A NAME="Article"></A>
author	Spollansky,Tatiana C. Pitta-Alvarez,Sandra I. Giulietti,Ana M.
spellingShingle	Spollansky,Tatiana C. Pitta-Alvarez,Sandra I. Giulietti,Ana M. Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida
author_facet	Spollansky,Tatiana C. Pitta-Alvarez,Sandra I. Giulietti,Ana M.
author_sort	Spollansky,Tatiana C.
title	Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida
title_short	Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida
title_full	Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida
title_fullStr	Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida
title_full_unstemmed	Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida
title_sort	effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of brugmansia candida
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000100006
work_keys_str_mv	AT spollanskytatianac effectofjasmonicacidandaluminiumonproductionoftropanealkaloidsinhairyrootculturesofbrugmansiacandida AT pittaalvarezsandrai effectofjasmonicacidandaluminiumonproductionoftropanealkaloidsinhairyrootculturesofbrugmansiacandida AT giuliettianam effectofjasmonicacidandaluminiumonproductionoftropanealkaloidsinhairyrootculturesofbrugmansiacandida
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Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candida

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