Plant genomic instability detected by microsatellite-primers
This report describes a new application of the Inter-Simple Sequence Repeat (ISSR) technique. This technology based on the amplification of regions between microsatellites was applied to different calli from the same cauliflower mother plant. One of the tested ISSR primers, (GATA)4, generated great...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2000
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oai:scielo:S0717-345820000002000052003-10-13Plant genomic instability detected by microsatellite-primersLeroy,Xavier J.Leon,KarineBranchard,Michel This report describes a new application of the Inter-Simple Sequence Repeat (ISSR) technique. This technology based on the amplification of regions between microsatellites was applied to different calli from the same cauliflower mother plant. One of the tested ISSR primers, (GATA)4, generated great polymorphism. Twelve different markers were detected on polyacrylamide gels. After sequencing, one sequence showed homology with a predicated A. thaliana gene closely related to genes involved in the regulation of cell proliferation in mammalians. This marker is characterised by three microsatellites and a palindromic sequence. Possible causes of mutations in this marker are discussed and will be investigated. ISSR amplification appears as a reliable method in the determination of genetic instability at early stages in in vitro culture. <A NAME="Article"></A>info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.3 n.2 20002000-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200005en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
This report describes a new application of the Inter-Simple Sequence Repeat (ISSR) technique. This technology based on the amplification of regions between microsatellites was applied to different calli from the same cauliflower mother plant. One of the tested ISSR primers, (GATA)4, generated great polymorphism. Twelve different markers were detected on polyacrylamide gels. After sequencing, one sequence showed homology with a predicated A. thaliana gene closely related to genes involved in the regulation of cell proliferation in mammalians. This marker is characterised by three microsatellites and a palindromic sequence. Possible causes of mutations in this marker are discussed and will be investigated. ISSR amplification appears as a reliable method in the determination of genetic instability at early stages in in vitro culture. <A NAME="Article"></A> |
| author |
Leroy,Xavier J. Leon,Karine Branchard,Michel |
| spellingShingle |
Leroy,Xavier J. Leon,Karine Branchard,Michel Plant genomic instability detected by microsatellite-primers |
| author_facet |
Leroy,Xavier J. Leon,Karine Branchard,Michel |
| author_sort |
Leroy,Xavier J. |
| title |
Plant genomic instability detected by microsatellite-primers |
| title_short |
Plant genomic instability detected by microsatellite-primers |
| title_full |
Plant genomic instability detected by microsatellite-primers |
| title_fullStr |
Plant genomic instability detected by microsatellite-primers |
| title_full_unstemmed |
Plant genomic instability detected by microsatellite-primers |
| title_sort |
plant genomic instability detected by microsatellite-primers |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2000 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200005 |
| work_keys_str_mv |
AT leroyxavierj plantgenomicinstabilitydetectedbymicrosatelliteprimers AT leonkarine plantgenomicinstabilitydetectedbymicrosatelliteprimers AT branchardmichel plantgenomicinstabilitydetectedbymicrosatelliteprimers |
| _version_ |
1718441689523159040 |