Protocol for regeneration in vitro of Arachis hypogaea L

The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultiv...

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Autores principales: Cucco,María Florencia, Rossi Jaume,Alberto Daniel
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2000
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200007
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spelling oai:scielo:S0717-345820000002000072003-10-13Protocol for regeneration in vitro of Arachis hypogaea LCucco,María FlorenciaRossi Jaume,Alberto Daniel The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.3 n.2 20002000-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200007en
institution Scielo Chile
collection Scielo Chile
language English
description The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage.
author Cucco,María Florencia
Rossi Jaume,Alberto Daniel
spellingShingle Cucco,María Florencia
Rossi Jaume,Alberto Daniel
Protocol for regeneration in vitro of Arachis hypogaea L
author_facet Cucco,María Florencia
Rossi Jaume,Alberto Daniel
author_sort Cucco,María Florencia
title Protocol for regeneration in vitro of Arachis hypogaea L
title_short Protocol for regeneration in vitro of Arachis hypogaea L
title_full Protocol for regeneration in vitro of Arachis hypogaea L
title_fullStr Protocol for regeneration in vitro of Arachis hypogaea L
title_full_unstemmed Protocol for regeneration in vitro of Arachis hypogaea L
title_sort protocol for regeneration in vitro of arachis hypogaea l
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2000
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200007
work_keys_str_mv AT cuccomariaflorencia protocolforregenerationinvitroofarachishypogaeal
AT rossijaumealbertodaniel protocolforregenerationinvitroofarachishypogaeal
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