Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli

Dividing cells of leaves used as sources of explants from coffee plants (Coffea arabica cv. 'Catimor') and those of their derived calli were analyzed for mitotic aberrations. The studied tissues were prepared by squashing and stained with carbolfuchsin. A total of 1551 leaf and 4568 callus...

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Autores principales:	Menéndez-Yuffá,Andrea, Fernandez Da Silva,Rafael, Rios,Liliana, Xena de Enrech,Nereida
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2000
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200008
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spelling	oai:scielo:S0717-345820000002000082003-10-13Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calliMenéndez-Yuffá,AndreaFernandez Da Silva,RafaelRios,LilianaXena de Enrech,Nereida Dividing cells of leaves used as sources of explants from coffee plants (Coffea arabica cv. 'Catimor') and those of their derived calli were analyzed for mitotic aberrations. The studied tissues were prepared by squashing and stained with carbolfuchsin. A total of 1551 leaf and 4568 callus cells were surveyed. The majority (79 %) of leaf and calli (75 %) cells showed normal mitosis, however, cells with mitotic abnormalities were also found in both tissues. These included: polyploids, aneuploids, sticky chromosomes, double prophases and lagging chromosomes. Additionally, interphase cells with micronuclei or binucleated were also observed. The frequencies of these abnormalities were statistically different in calli and leaves. Calli showed a few other abnormalities such as c-mitosis, chained chromosomes, multipolar metaphases and chromosome bridges. Therefore, we conclude that these pre-existing abnormalities originate by errors in the process of normal mitosis in both leaves and in calli, and are therefore not caused by tissue culture conditions. <A NAME="Article"></A>info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.3 n.2 20002000-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200008en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Dividing cells of leaves used as sources of explants from coffee plants (Coffea arabica cv. 'Catimor') and those of their derived calli were analyzed for mitotic aberrations. The studied tissues were prepared by squashing and stained with carbolfuchsin. A total of 1551 leaf and 4568 callus cells were surveyed. The majority (79 %) of leaf and calli (75 %) cells showed normal mitosis, however, cells with mitotic abnormalities were also found in both tissues. These included: polyploids, aneuploids, sticky chromosomes, double prophases and lagging chromosomes. Additionally, interphase cells with micronuclei or binucleated were also observed. The frequencies of these abnormalities were statistically different in calli and leaves. Calli showed a few other abnormalities such as c-mitosis, chained chromosomes, multipolar metaphases and chromosome bridges. Therefore, we conclude that these pre-existing abnormalities originate by errors in the process of normal mitosis in both leaves and in calli, and are therefore not caused by tissue culture conditions. <A NAME="Article"></A>
author	Menéndez-Yuffá,Andrea Fernandez Da Silva,Rafael Rios,Liliana Xena de Enrech,Nereida
spellingShingle	Menéndez-Yuffá,Andrea Fernandez Da Silva,Rafael Rios,Liliana Xena de Enrech,Nereida Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli
author_facet	Menéndez-Yuffá,Andrea Fernandez Da Silva,Rafael Rios,Liliana Xena de Enrech,Nereida
author_sort	Menéndez-Yuffá,Andrea
title	Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli
title_short	Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli
title_full	Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli
title_fullStr	Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli
title_full_unstemmed	Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli
title_sort	mitotic aberrations in coffee (coffea arabica cv. 'catimor') leaf explants and their derived embryogenic calli
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000200008
work_keys_str_mv	AT menendezyuffaandrea mitoticaberrationsincoffeecoffeaarabicacvcatimorleafexplantsandtheirderivedembryogeniccalli AT fernandezdasilvarafael mitoticaberrationsincoffeecoffeaarabicacvcatimorleafexplantsandtheirderivedembryogeniccalli AT riosliliana mitoticaberrationsincoffeecoffeaarabicacvcatimorleafexplantsandtheirderivedembryogeniccalli AT xenadeenrechnereida mitoticaberrationsincoffeecoffeaarabicacvcatimorleafexplantsandtheirderivedembryogeniccalli
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Mitotic aberrations in coffee (Coffea arabica cv. 'Catimor') leaf explants and their derived embryogenic calli

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