Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates

Calcitonin gene related peptide (CGRP) related molecules were purified from sardine hydrolysates prepared using 0.1% alcalase and two hours of hydrolysis. Gel exclusion chromatography and HPLC performed purification of these molecules. The purified molecules were characterised using specific CGRP ra...

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Autores principales:	Rousseau,Marthe, Batista,Irineu, Le Gal,Yves, Fouchereau-Peron,Martine
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2001
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100003
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spelling	oai:scielo:S0717-345820010001000032005-12-12Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysatesRousseau,MartheBatista,IrineuLe Gal,YvesFouchereau-Peron,Martine Calcitonin gene related peptide (CGRP) related molecules were purified from sardine hydrolysates prepared using 0.1% alcalase and two hours of hydrolysis. Gel exclusion chromatography and HPLC performed purification of these molecules. The purified molecules were characterised using specific CGRP radioimmunoassays and radioreceptoraasays. From 22 mg of crude extract, we obtained 14 µg of CGRP related molecules, the molecular weight determined by mass spectrophotometry was 6000 daltons. The biological activity of these molecules was analysed using the ability of CGRP to stimulate the adenylate cyclase activity in rat liver membranes. The purified molecules induced an inhibition of the CGRP stimulated adenylate cyclase activity, this effect was specific as no such effect was observed on the glucagon stimulated adenylate cyclase activity measured in the same rat liver membrane preparation. These results suggest that the purified molecules may act as antagonists for peptides that bind to CGRP receptors in rat liver membranes. These new antagonists may be of particular importance in various aspects of CGRP action in vertebrates. <A NAME="Article"></A>info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.4 n.1 20012001-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100003en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Calcitonin gene related peptide (CGRP) related molecules were purified from sardine hydrolysates prepared using 0.1% alcalase and two hours of hydrolysis. Gel exclusion chromatography and HPLC performed purification of these molecules. The purified molecules were characterised using specific CGRP radioimmunoassays and radioreceptoraasays. From 22 mg of crude extract, we obtained 14 µg of CGRP related molecules, the molecular weight determined by mass spectrophotometry was 6000 daltons. The biological activity of these molecules was analysed using the ability of CGRP to stimulate the adenylate cyclase activity in rat liver membranes. The purified molecules induced an inhibition of the CGRP stimulated adenylate cyclase activity, this effect was specific as no such effect was observed on the glucagon stimulated adenylate cyclase activity measured in the same rat liver membrane preparation. These results suggest that the purified molecules may act as antagonists for peptides that bind to CGRP receptors in rat liver membranes. These new antagonists may be of particular importance in various aspects of CGRP action in vertebrates. <A NAME="Article"></A>
author	Rousseau,Marthe Batista,Irineu Le Gal,Yves Fouchereau-Peron,Martine
spellingShingle	Rousseau,Marthe Batista,Irineu Le Gal,Yves Fouchereau-Peron,Martine Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates
author_facet	Rousseau,Marthe Batista,Irineu Le Gal,Yves Fouchereau-Peron,Martine
author_sort	Rousseau,Marthe
title	Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates
title_short	Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates
title_full	Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates
title_fullStr	Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates
title_full_unstemmed	Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates
title_sort	purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100003
work_keys_str_mv	AT rousseaumarthe purificationofafunctionalcompetitiveantagonistforcalcitoningenerelatedpeptideactionfromsardinehydrolysates AT batistairineu purificationofafunctionalcompetitiveantagonistforcalcitoningenerelatedpeptideactionfromsardinehydrolysates AT legalyves purificationofafunctionalcompetitiveantagonistforcalcitoningenerelatedpeptideactionfromsardinehydrolysates AT fouchereauperonmartine purificationofafunctionalcompetitiveantagonistforcalcitoningenerelatedpeptideactionfromsardinehydrolysates
_version_	1718441692432957440

Purification of a functional competitive antagonist for calcitonin gene related peptide action from sardine hydrolysates

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