A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture

High-density cultures of mammalian neurons offer a model system for studies of brain development, but the morphological features of individual neurons is difficult to ascertain. We show that a herpes virus vector expressing a bioluminescent protein allows detailed morphometric analyses of living neu...

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Autores principales: Falk,Torsten, Strazdas,Lori A., Borders,Rebecca S., Kilani,Ramsey K., Yool,Andrea J., Sherman,Scott J.
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2001
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100004
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spelling oai:scielo:S0717-345820010001000042005-12-12A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal cultureFalk,TorstenStrazdas,Lori A.Borders,Rebecca S.Kilani,Ramsey K.Yool,Andrea J.Sherman,Scott J. High-density cultures of mammalian neurons offer a model system for studies of brain development, but the morphological features of individual neurons is difficult to ascertain. We show that a herpes virus vector expressing a bioluminescent protein allows detailed morphometric analyses of living neurons in complex culture environments. Expression of enhanced green fluorescent protein (eGFP) was constitutively driven in neurons using the herpes simplex virus amplicon system. This system allowed us to make novel observations regarding development in high-density cultures from rat hippocampus and cerebellum. After the phase of initial neurite outgrowth, maturing neurons continue to show rapid remodeling of the neurite branches (0.79 ± 0.11 mum/h per neurite; mean ± SEM, n=8), and displacement of the soma within the neurite arbor (1.35 ± 0.74 mum/h). These results demonstrate that a substantial capacity for morphological plasticity persists in maturing mammalian CNS neurons after cessation of net neurite outgrowth in early development. <A NAME="Article"></A>info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.4 n.1 20012001-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100004en
institution Scielo Chile
collection Scielo Chile
language English
description High-density cultures of mammalian neurons offer a model system for studies of brain development, but the morphological features of individual neurons is difficult to ascertain. We show that a herpes virus vector expressing a bioluminescent protein allows detailed morphometric analyses of living neurons in complex culture environments. Expression of enhanced green fluorescent protein (eGFP) was constitutively driven in neurons using the herpes simplex virus amplicon system. This system allowed us to make novel observations regarding development in high-density cultures from rat hippocampus and cerebellum. After the phase of initial neurite outgrowth, maturing neurons continue to show rapid remodeling of the neurite branches (0.79 ± 0.11 mum/h per neurite; mean ± SEM, n=8), and displacement of the soma within the neurite arbor (1.35 ± 0.74 mum/h). These results demonstrate that a substantial capacity for morphological plasticity persists in maturing mammalian CNS neurons after cessation of net neurite outgrowth in early development. <A NAME="Article"></A>
author Falk,Torsten
Strazdas,Lori A.
Borders,Rebecca S.
Kilani,Ramsey K.
Yool,Andrea J.
Sherman,Scott J.
spellingShingle Falk,Torsten
Strazdas,Lori A.
Borders,Rebecca S.
Kilani,Ramsey K.
Yool,Andrea J.
Sherman,Scott J.
A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
author_facet Falk,Torsten
Strazdas,Lori A.
Borders,Rebecca S.
Kilani,Ramsey K.
Yool,Andrea J.
Sherman,Scott J.
author_sort Falk,Torsten
title A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
title_short A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
title_full A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
title_fullStr A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
title_full_unstemmed A herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
title_sort herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal culture
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2001
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100004
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