Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor

A protease inhibitor gene has been isolated from native cowpea (Vigna unguiculata L.) cv. 130 from lambda-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lawrence,Paulraj Kirubakaran, Nirmala,Jayaveeramuthu, Koundal,Kripa Ram
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2001
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100005
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582001000100005
record_format	dspace
spelling	oai:scielo:S0717-345820010001000052005-12-12Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitorLawrence,Paulraj KirubakaranNirmala,JayaveeramuthuKoundal,Kripa Ram A protease inhibitor gene has been isolated from native cowpea (Vigna unguiculata L.) cv. 130 from lambda-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean. The isolated gene had TATA and CAT signals in the 5' upstream region. The longest open reading frame had 504 bases, encoding 167 amino acids from the predicted coding region. The active serine centers of this protein was found between 105-158 amino acid residues, with a 69 amino acids long signal peptide in its N terminal. <A NAME="Article"></A>info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.4 n.1 20012001-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100005en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	A protease inhibitor gene has been isolated from native cowpea (Vigna unguiculata L.) cv. 130 from lambda-ZAP II genomic library. The nucleotide sequence of this genomic clone shared 86% homology with a cowpea trypsin inhibitor f IV mRNA, and 81% with Bowman-Birk protease inhibitor genes of soybean. The isolated gene had TATA and CAT signals in the 5' upstream region. The longest open reading frame had 504 bases, encoding 167 amino acids from the predicted coding region. The active serine centers of this protein was found between 105-158 amino acid residues, with a 69 amino acids long signal peptide in its N terminal. <A NAME="Article"></A>
author	Lawrence,Paulraj Kirubakaran Nirmala,Jayaveeramuthu Koundal,Kripa Ram
spellingShingle	Lawrence,Paulraj Kirubakaran Nirmala,Jayaveeramuthu Koundal,Kripa Ram Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor
author_facet	Lawrence,Paulraj Kirubakaran Nirmala,Jayaveeramuthu Koundal,Kripa Ram
author_sort	Lawrence,Paulraj Kirubakaran
title	Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor
title_short	Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor
title_full	Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor
title_fullStr	Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor
title_full_unstemmed	Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor
title_sort	nucleotide sequence of a genomic clone encoding a cowpea (vigna unguiculata l.) trypsin inhibitor
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000100005
work_keys_str_mv	AT lawrencepaulrajkirubakaran nucleotidesequenceofagenomiccloneencodingacowpeavignaunguiculataltrypsininhibitor AT nirmalajayaveeramuthu nucleotidesequenceofagenomiccloneencodingacowpeavignaunguiculataltrypsininhibitor AT koundalkriparam nucleotidesequenceofagenomiccloneencodingacowpeavignaunguiculataltrypsininhibitor
_version_	1718441693008625664

Nucleotide sequence of a genomic clone encoding a cowpea (Vigna unguiculata L.) trypsin inhibitor

Ejemplares similares