Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)

We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to identify cDNAs corresponding to transcripts affected by water stress in peanuts (Arachis hypogaea L.). Using this method, we have identified several mRNA transcripts that are up- or down-regulated foll...

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Autores principales:	Jain,Ashok K., Basha,Sheikh Mehboob, Holbrook,C. Corley
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2001
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200005
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spelling	oai:scielo:S0717-345820010002000052003-08-18Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)Jain,Ashok K.Basha,Sheikh MehboobHolbrook,C. Corley We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to identify cDNAs corresponding to transcripts affected by water stress in peanuts (Arachis hypogaea L.). Using this method, we have identified several mRNA transcripts that are up- or down-regulated following water stress. With 21 primer combinations, a total of 1235 differential display products were observed in irrigated samples, compared to 950 differential display products in stressed samples. These products demonstrated qualitative and quantitative differences in the gene expression. The differentially expressed transcripts were collectively named PTRD (Peanut Transcripts Responsive to Drought). We have identified a total of 43 PTRD, which were significantly altered due to water stress. Slot blot analysis of 16 PTRD indicated that 12 were completely suppressed due to prolonged drought, two were down-regulated, and two were up-regulated under drought stress conditions. The 12 completely suppressed transcripts were studied further by RNA dot-blot analysis to compare their expression in drought tolerant and susceptible lines, which underwent three weeks of water stress. PTRD-1, -10, and -16 expressed for longer period in tolerant line compared to the susceptible line and can be used as molecular markers for screening peanut lines for drought toleranceinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.4 n.2 20012001-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200005en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to identify cDNAs corresponding to transcripts affected by water stress in peanuts (Arachis hypogaea L.). Using this method, we have identified several mRNA transcripts that are up- or down-regulated following water stress. With 21 primer combinations, a total of 1235 differential display products were observed in irrigated samples, compared to 950 differential display products in stressed samples. These products demonstrated qualitative and quantitative differences in the gene expression. The differentially expressed transcripts were collectively named PTRD (Peanut Transcripts Responsive to Drought). We have identified a total of 43 PTRD, which were significantly altered due to water stress. Slot blot analysis of 16 PTRD indicated that 12 were completely suppressed due to prolonged drought, two were down-regulated, and two were up-regulated under drought stress conditions. The 12 completely suppressed transcripts were studied further by RNA dot-blot analysis to compare their expression in drought tolerant and susceptible lines, which underwent three weeks of water stress. PTRD-1, -10, and -16 expressed for longer period in tolerant line compared to the susceptible line and can be used as molecular markers for screening peanut lines for drought tolerance
author	Jain,Ashok K. Basha,Sheikh Mehboob Holbrook,C. Corley
spellingShingle	Jain,Ashok K. Basha,Sheikh Mehboob Holbrook,C. Corley Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)
author_facet	Jain,Ashok K. Basha,Sheikh Mehboob Holbrook,C. Corley
author_sort	Jain,Ashok K.
title	Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)
title_short	Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)
title_full	Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)
title_fullStr	Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)
title_full_unstemmed	Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)
title_sort	identification of drought-responsive transcripts in peanut (arachis hypogaea l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200005
work_keys_str_mv	AT jainashokk identificationofdroughtresponsivetranscriptsinpeanutarachishypogaeal AT bashasheikhmehboob identificationofdroughtresponsivetranscriptsinpeanutarachishypogaeal AT holbrookccorley identificationofdroughtresponsivetranscriptsinpeanutarachishypogaeal
_version_	1718441694136893440

Identification of drought-responsive transcripts in peanut (Arachis hypogaea L.)

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