Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize

The well-known entomopathogenic bacterium Bacillus thuringiensis (Bt) produces a spore-crystal complex which is responsible for its biocide characteristic, and the bacterium can be obtained by fermentation, either in liquid or semi-solid substrates. This paper presents a successful way to achieve so...

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Autores principales:	Fontana Capalbo,Deise Maria, Valicente,Fernando Hercule, Oliveira Moraes,Iracema de, Pelizer,Lúcia Helena
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2001
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200009
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spelling	oai:scielo:S0717-345820010002000092003-08-18Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maizeFontana Capalbo,Deise MariaValicente,Fernando HerculeOliveira Moraes,Iracema dePelizer,Lúcia Helena The well-known entomopathogenic bacterium Bacillus thuringiensis (Bt) produces a spore-crystal complex which is responsible for its biocide characteristic, and the bacterium can be obtained by fermentation, either in liquid or semi-solid substrates. This paper presents a successful way to achieve solid-state fermentation of active Bt var. tolworthi (Btt) against Spodoptera frugiperda (fall armyworm) in corn. More than 10(9) CFU/g were obtained using humidified rice as substrate maintained in polypropylene bags. This active complex (substrate plus spore-crystal of Bt) was prepared in order to obtain 2 x 10(6) spores/mL; the final suspension then sprayed via tractor on corn fields. On the treated plants, mortality of neonate larvae was 100% within two days of spraying, and all larvae were found dead on leaves. During one maize crop cycle, two applications were made, and up until 70 days after emergence it was not necessary to apply any other insecticide for fall armyworm control.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.4 n.2 20012001-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200009en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	The well-known entomopathogenic bacterium Bacillus thuringiensis (Bt) produces a spore-crystal complex which is responsible for its biocide characteristic, and the bacterium can be obtained by fermentation, either in liquid or semi-solid substrates. This paper presents a successful way to achieve solid-state fermentation of active Bt var. tolworthi (Btt) against Spodoptera frugiperda (fall armyworm) in corn. More than 10(9) CFU/g were obtained using humidified rice as substrate maintained in polypropylene bags. This active complex (substrate plus spore-crystal of Bt) was prepared in order to obtain 2 x 10(6) spores/mL; the final suspension then sprayed via tractor on corn fields. On the treated plants, mortality of neonate larvae was 100% within two days of spraying, and all larvae were found dead on leaves. During one maize crop cycle, two applications were made, and up until 70 days after emergence it was not necessary to apply any other insecticide for fall armyworm control.
author	Fontana Capalbo,Deise Maria Valicente,Fernando Hercule Oliveira Moraes,Iracema de Pelizer,Lúcia Helena
spellingShingle	Fontana Capalbo,Deise Maria Valicente,Fernando Hercule Oliveira Moraes,Iracema de Pelizer,Lúcia Helena Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize
author_facet	Fontana Capalbo,Deise Maria Valicente,Fernando Hercule Oliveira Moraes,Iracema de Pelizer,Lúcia Helena
author_sort	Fontana Capalbo,Deise Maria
title	Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize
title_short	Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize
title_full	Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize
title_fullStr	Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize
title_full_unstemmed	Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize
title_sort	solid-state fermentation of bacillus thuringiensis tolworthi to control fall armyworm in maize
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000200009
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_version_	1718441695201198080

Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize

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