Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier
A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved therm...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2001
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000300006 |
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oai:scielo:S0717-345820010003000062003-08-18Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrierAfaq,SarahIqbal,Jawaid A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved thermal stability and the carrier could be recovered from the spent bound enzyme, to be reused. Reimmobilization of papain on the regenerated matrix was equally effective with the retention of maximum enzyme activity.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.4 n.3 20012001-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000300006en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
A method for immobilization of papain has been selected based on the interaction between its histidine, cysteine and tryptophan residues with the immobilized metal ion (IMI) carrier for maximum binding on a small volume of the carrier. The immobilized papain retained high activity has improved thermal stability and the carrier could be recovered from the spent bound enzyme, to be reused. Reimmobilization of papain on the regenerated matrix was equally effective with the retention of maximum enzyme activity. |
| author |
Afaq,Sarah Iqbal,Jawaid |
| spellingShingle |
Afaq,Sarah Iqbal,Jawaid Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier |
| author_facet |
Afaq,Sarah Iqbal,Jawaid |
| author_sort |
Afaq,Sarah |
| title |
Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier |
| title_short |
Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier |
| title_full |
Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier |
| title_fullStr |
Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier |
| title_full_unstemmed |
Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier |
| title_sort |
immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2001 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000300006 |
| work_keys_str_mv |
AT afaqsarah immobilizationandstabilizationofpapainonchelatingsepharoseametalchelateregenerablecarrier AT iqbaljawaid immobilizationandstabilizationofpapainonchelatingsepharoseametalchelateregenerablecarrier |
| _version_ |
1718441696980631552 |