Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test

Microsatellite polymorphism analysis on 25 different "Sangiovese" accessions was carried out at eight microsatellite loci (VVS2, VVS4, VVS29, VVMD3, VVMD6, VVMD7, VVMD17 and VVMD21). In order to evaluate variability within the "Sangiovese" variety and to confirm variety identific...

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Autores principales:	Vignani,Rita, Scali,Monica, Masi,Elisa, Cresti,Mauro
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100007
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spelling	oai:scielo:S0717-345820020001000072003-08-18Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP testVignani,RitaScali,MonicaMasi,ElisaCresti,Mauro Microsatellite polymorphism analysis on 25 different "Sangiovese" accessions was carried out at eight microsatellite loci (VVS2, VVS4, VVS29, VVMD3, VVMD6, VVMD7, VVMD17 and VVMD21). In order to evaluate variability within the "Sangiovese" variety and to confirm variety identification, genotype analysis, allele distribution and pedigree information were processed with a DNA-automated sequencer running AlleleLinks software. DNA typing revealed three cases of genetic dissimilarity compared to registered "Sangiovese" clones and the divergent accessions thought to be different clones of the same variety. The divergent accessions were different from "Sangiovese" at four microsatellite loci (VVS2, VVS4, VVMD7 and VVMD21). An innovative non-radioactive modification of AFLP genome profiling confirmed the data obtained by microsatellite amplification test. These results underline the importance of biotechnological testing, such as the PCR-based DNA tests together with traditional ampelography, in Vitis vinifera L. clone and variety selection programmes to avoid misnaming and erroneous identification and to evaluate genetic relatedness and variability within populationsinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.1 20022002-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100007en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Microsatellite polymorphism analysis on 25 different "Sangiovese" accessions was carried out at eight microsatellite loci (VVS2, VVS4, VVS29, VVMD3, VVMD6, VVMD7, VVMD17 and VVMD21). In order to evaluate variability within the "Sangiovese" variety and to confirm variety identification, genotype analysis, allele distribution and pedigree information were processed with a DNA-automated sequencer running AlleleLinks software. DNA typing revealed three cases of genetic dissimilarity compared to registered "Sangiovese" clones and the divergent accessions thought to be different clones of the same variety. The divergent accessions were different from "Sangiovese" at four microsatellite loci (VVS2, VVS4, VVMD7 and VVMD21). An innovative non-radioactive modification of AFLP genome profiling confirmed the data obtained by microsatellite amplification test. These results underline the importance of biotechnological testing, such as the PCR-based DNA tests together with traditional ampelography, in Vitis vinifera L. clone and variety selection programmes to avoid misnaming and erroneous identification and to evaluate genetic relatedness and variability within populations
author	Vignani,Rita Scali,Monica Masi,Elisa Cresti,Mauro
spellingShingle	Vignani,Rita Scali,Monica Masi,Elisa Cresti,Mauro Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test
author_facet	Vignani,Rita Scali,Monica Masi,Elisa Cresti,Mauro
author_sort	Vignani,Rita
title	Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test
title_short	Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test
title_full	Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test
title_fullStr	Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test
title_full_unstemmed	Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test
title_sort	genomic variability in vitis vinifera l. "sangiovese" assessed by microsatellite and non-radioactive aflp test
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100007
work_keys_str_mv	AT vignanirita genomicvariabilityinvitisviniferalsangioveseassessedbymicrosatelliteandnonradioactiveaflptest AT scalimonica genomicvariabilityinvitisviniferalsangioveseassessedbymicrosatelliteandnonradioactiveaflptest AT masielisa genomicvariabilityinvitisviniferalsangioveseassessedbymicrosatelliteandnonradioactiveaflptest AT crestimauro genomicvariabilityinvitisviniferalsangioveseassessedbymicrosatelliteandnonradioactiveaflptest
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Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test

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