Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene

The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens...

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Autores principales:	Sun,Chuan-Bao, Kong,Qiu-Lian, Xu,Wen-Si
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100009
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spelling	oai:scielo:S0717-345820020001000092003-08-18Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin geneSun,Chuan-BaoKong,Qiu-LianXu,Wen-Si The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.1 20022002-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100009en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts.
author	Sun,Chuan-Bao Kong,Qiu-Lian Xu,Wen-Si
spellingShingle	Sun,Chuan-Bao Kong,Qiu-Lian Xu,Wen-Si Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
author_facet	Sun,Chuan-Bao Kong,Qiu-Lian Xu,Wen-Si
author_sort	Sun,Chuan-Bao
title	Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
title_short	Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
title_full	Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
title_fullStr	Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
title_full_unstemmed	Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
title_sort	efficient transformation of penicillium chrysogenum mediated by agrobacterium tumefaciens lba4404 for cloning of vitreoscilla hemoglobin gene
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100009
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Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene

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