Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity

Single Strand-Preferring Nucleases (SSPNs) have been implicated in the triggering and progress of cell death pathways, including senescence in higher plants, though the biological roles of such enzymes are still obscure. In the present study, heterotrophic cell suspension cultures of Oryza sativa L....

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Autores principales:	Hosseini,Ramin, Mulligan,Bernard J.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100011
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spelling	oai:scielo:S0717-345820020001000112003-08-18Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activityHosseini,RaminMulligan,Bernard J. Single Strand-Preferring Nucleases (SSPNs) have been implicated in the triggering and progress of cell death pathways, including senescence in higher plants, though the biological roles of such enzymes are still obscure. In the present study, heterotrophic cell suspension cultures of Oryza sativa L. (rice) cv Taipei 309 were used to investigate changes in Single Strand-Preferring Nuclease activity associated with cell death in vitro. An acid nuclease activity (pH 5.5) was found which was strongly stimulated in the presence of 10 mM Zn2+ and inhibited by 10 mM EDTA and EGTA. An increase in SSPN activity was concomitant with a loss of cell viability, total protein and the onset of stationary phase of growth in the cell cultures. Using DNA-SDS-PAGE two major SSPNs were detected with the molecular weights of 26 KDa and 53.5 KDa. There are possibilities that these two enzymes represent monomeric and dimeric forms of the same nuclease or they are two different enzymes. The 26 KDa nuclease was partially purified using heparin-Sepharose column chromatography. The results confirm the value of plant cell suspension cultures for the investigation of the molecular process underlying plant cell ageing, senescence processes and programmed cell death (PCD). Possible senescence-associated roles of SSPNs are discussed.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.1 20022002-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100011en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Single Strand-Preferring Nucleases (SSPNs) have been implicated in the triggering and progress of cell death pathways, including senescence in higher plants, though the biological roles of such enzymes are still obscure. In the present study, heterotrophic cell suspension cultures of Oryza sativa L. (rice) cv Taipei 309 were used to investigate changes in Single Strand-Preferring Nuclease activity associated with cell death in vitro. An acid nuclease activity (pH 5.5) was found which was strongly stimulated in the presence of 10 mM Zn2+ and inhibited by 10 mM EDTA and EGTA. An increase in SSPN activity was concomitant with a loss of cell viability, total protein and the onset of stationary phase of growth in the cell cultures. Using DNA-SDS-PAGE two major SSPNs were detected with the molecular weights of 26 KDa and 53.5 KDa. There are possibilities that these two enzymes represent monomeric and dimeric forms of the same nuclease or they are two different enzymes. The 26 KDa nuclease was partially purified using heparin-Sepharose column chromatography. The results confirm the value of plant cell suspension cultures for the investigation of the molecular process underlying plant cell ageing, senescence processes and programmed cell death (PCD). Possible senescence-associated roles of SSPNs are discussed.
author	Hosseini,Ramin Mulligan,Bernard J.
spellingShingle	Hosseini,Ramin Mulligan,Bernard J. Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity
author_facet	Hosseini,Ramin Mulligan,Bernard J.
author_sort	Hosseini,Ramin
title	Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity
title_short	Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity
title_full	Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity
title_fullStr	Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity
title_full_unstemmed	Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity
title_sort	application of rice (oryza sativa l.) suspension culture in studying senescence in vitro (i).: single strand preferring nuclease activity
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100011
work_keys_str_mv	AT hosseiniramin applicationofriceoryzasativalsuspensioncultureinstudyingsenescenceinvitroisinglestrandpreferringnucleaseactivity AT mulliganbernardj applicationofriceoryzasativalsuspensioncultureinstudyingsenescenceinvitroisinglestrandpreferringnucleaseactivity
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Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I).: Single strand preferring nuclease activity

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