Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity

Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity

Changes in the DNA content and organisation of senescing rice cell cultures (Taipei 309) were studied, using PCR and Southern blot analyses. A mitochondrial gene (coxII), a plastid gene (psaA) and a nuclear DNA maker (RG64) were analysed. The amplification of mitochondrial (mt), plastid and nuclear...

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Autores principales: Ramin,Hosseini, Mulligan,Bernard J.
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100012
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spelling oai:scielo:S0717-345820020001000122003-10-17Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrityRamin,HosseiniMulligan,Bernard J. Changes in the DNA content and organisation of senescing rice cell cultures (Taipei 309) were studied, using PCR and Southern blot analyses. A mitochondrial gene (coxII), a plastid gene (psaA) and a nuclear DNA maker (RG64) were analysed. The amplification of mitochondrial (mt), plastid and nuclear DNA produced the expected fragments, indicating that there were still some intact organelles and nuclei in the senescing rice cells. However, in plastid and nuclear DNA, changes in the number and size of the PCR products were observed. Southern blot analysis revealed that mt and nuclear DNA contents declined, but plastid DNA content remained relatively the same throughout the senescence.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.1 20022002-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100012en
institution Scielo Chile
collection Scielo Chile
language English
description Changes in the DNA content and organisation of senescing rice cell cultures (Taipei 309) were studied, using PCR and Southern blot analyses. A mitochondrial gene (coxII), a plastid gene (psaA) and a nuclear DNA maker (RG64) were analysed. The amplification of mitochondrial (mt), plastid and nuclear DNA produced the expected fragments, indicating that there were still some intact organelles and nuclei in the senescing rice cells. However, in plastid and nuclear DNA, changes in the number and size of the PCR products were observed. Southern blot analysis revealed that mt and nuclear DNA contents declined, but plastid DNA content remained relatively the same throughout the senescence.
author Ramin,Hosseini
Mulligan,Bernard J.
spellingShingle Ramin,Hosseini
Mulligan,Bernard J.
Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity
author_facet Ramin,Hosseini
Mulligan,Bernard J.
author_sort Ramin,Hosseini
title Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity
title_short Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity
title_full Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity
title_fullStr Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity
title_full_unstemmed Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (II).: Changes in DNA integrity
title_sort application of rice (oryza sativa l.) suspension culture in studying senescence in vitro (ii).: changes in dna integrity
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2002
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100012
work_keys_str_mv AT raminhosseini applicationofriceoryzasativalsuspensioncultureinstudyingsenescenceinvitroiichangesindnaintegrity
AT mulliganbernardj applicationofriceoryzasativalsuspensioncultureinstudyingsenescenceinvitroiichangesindnaintegrity
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