Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae

L-DOPA is a useful drug for Parkinsons disease. This investigation deals with the biosynthesis of L-DOPA by parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae. There was a marked difference between the mycelial morphology and pellet type of the parental and UV-irradiated mutant cultur...

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Autores principales:	Ali,Sikander, ul-Haq,Ikram, Qadeer,M.A.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200006
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spelling	oai:scielo:S0717-345820020002000062003-08-19Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzaeAli,Sikanderul-Haq,IkramQadeer,M.A. L-DOPA is a useful drug for Parkinsons disease. This investigation deals with the biosynthesis of L-DOPA by parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae. There was a marked difference between the mycelial morphology and pellet type of the parental and UV-irradiated mutant cultures. The mutant strain of Aspergillus oryzae UV-6 showed pellet-like mycelial morphology and improved tyrosinase activity. Mould mycelium was used for biochemical conversion of L-tyrosine to L-DOPA since tyrosinase is an intracellular enzyme. The mutant was found to give 3.72 folds higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed greater ability of the mutant to yield L-DOPA (i.e. Yp/x 32.73 mg/mg with parent and 95.71 mg/mg with mutant). When cultures grown for various incubation periods, were monitored for Qp, Qs and q p, there was significant enhancement (p<0.0025-0.005) in these variables by the mutant strain of Aspergillus oryzae UV-7 over GCB-6 on all the rates.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.2 20022002-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200006en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	L-DOPA is a useful drug for Parkinsons disease. This investigation deals with the biosynthesis of L-DOPA by parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae. There was a marked difference between the mycelial morphology and pellet type of the parental and UV-irradiated mutant cultures. The mutant strain of Aspergillus oryzae UV-6 showed pellet-like mycelial morphology and improved tyrosinase activity. Mould mycelium was used for biochemical conversion of L-tyrosine to L-DOPA since tyrosinase is an intracellular enzyme. The mutant was found to give 3.72 folds higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed greater ability of the mutant to yield L-DOPA (i.e. Yp/x 32.73 mg/mg with parent and 95.71 mg/mg with mutant). When cultures grown for various incubation periods, were monitored for Qp, Qs and q p, there was significant enhancement (p<0.0025-0.005) in these variables by the mutant strain of Aspergillus oryzae UV-7 over GCB-6 on all the rates.
author	Ali,Sikander ul-Haq,Ikram Qadeer,M.A.
spellingShingle	Ali,Sikander ul-Haq,Ikram Qadeer,M.A. Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae
author_facet	Ali,Sikander ul-Haq,Ikram Qadeer,M.A.
author_sort	Ali,Sikander
title	Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae
title_short	Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae
title_full	Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae
title_fullStr	Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae
title_full_unstemmed	Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae
title_sort	novel technique for microbial production of 3,4-dihydroxy phenyl l-alanine by a mutant strain of aspergillus oryzae
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200006
work_keys_str_mv	AT alisikander noveltechniqueformicrobialproductionof34dihydroxyphenyllalaninebyamutantstrainofaspergillusoryzae AT ulhaqikram noveltechniqueformicrobialproductionof34dihydroxyphenyllalaninebyamutantstrainofaspergillusoryzae AT qadeerma noveltechniqueformicrobialproductionof34dihydroxyphenyllalaninebyamutantstrainofaspergillusoryzae
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Novel technique for microbial production of 3,4-Dihydroxy Phenyl L-alanine by a mutant strain of Aspergillus oryzae

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