Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium
Citric acid fermentation of cane-molasses by submerged fermentation in 15 L stirred fermentor (working volume 9 L) was carried out. A hyper mutant strain of Aspergillus niger GCMC-7 was used in the present study which was obtained from the culture collection of our own labs. Ferrocyanide treated mol...
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Pontificia Universidad Católica de Valparaíso
2002
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oai:scielo:S0717-345820020002000072003-08-19Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based mediumul-Haq,IkramAli,SikanderQadeer,M.A.Iqbal,Javed Citric acid fermentation of cane-molasses by submerged fermentation in 15 L stirred fermentor (working volume 9 L) was carried out. A hyper mutant strain of Aspergillus niger GCMC-7 was used in the present study which was obtained from the culture collection of our own labs. Ferrocyanide treated molasses [K4Fe(CN)6 200 ppm] medium containing sugar 150 g/l was employed as the basal fermentation medium. Different cultural conditions such as incubation temperature (30ºC), initial pH (6.0), air supply (1.0 l-1l-1min), agitation intensity (200 rpm) and time profile (144 h after inoculation) were optimised for enhanced citric acid production. Maximum amount of anhydrous citric acid obtained during the course of study was 106.65 g/l, with a sugar consumption of 107 g/l. Final pH, ferrocyanide concentration and dry cell mass were 2.1, 60 ppm and 16.5 g/l, respectively.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.2 20022002-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200007en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
Citric acid fermentation of cane-molasses by submerged fermentation in 15 L stirred fermentor (working volume 9 L) was carried out. A hyper mutant strain of Aspergillus niger GCMC-7 was used in the present study which was obtained from the culture collection of our own labs. Ferrocyanide treated molasses [K4Fe(CN)6 200 ppm] medium containing sugar 150 g/l was employed as the basal fermentation medium. Different cultural conditions such as incubation temperature (30ºC), initial pH (6.0), air supply (1.0 l-1l-1min), agitation intensity (200 rpm) and time profile (144 h after inoculation) were optimised for enhanced citric acid production. Maximum amount of anhydrous citric acid obtained during the course of study was 106.65 g/l, with a sugar consumption of 107 g/l. Final pH, ferrocyanide concentration and dry cell mass were 2.1, 60 ppm and 16.5 g/l, respectively. |
| author |
ul-Haq,Ikram Ali,Sikander Qadeer,M.A. Iqbal,Javed |
| spellingShingle |
ul-Haq,Ikram Ali,Sikander Qadeer,M.A. Iqbal,Javed Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium |
| author_facet |
ul-Haq,Ikram Ali,Sikander Qadeer,M.A. Iqbal,Javed |
| author_sort |
ul-Haq,Ikram |
| title |
Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium |
| title_short |
Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium |
| title_full |
Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium |
| title_fullStr |
Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium |
| title_full_unstemmed |
Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium |
| title_sort |
citric acid fermentation by mutant strain of aspergillus niger gcmc-7using molasses based medium |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2002 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200007 |
| work_keys_str_mv |
AT ulhaqikram citricacidfermentationbymutantstrainofaspergillusnigergcmc7usingmolassesbasedmedium AT alisikander citricacidfermentationbymutantstrainofaspergillusnigergcmc7usingmolassesbasedmedium AT qadeerma citricacidfermentationbymutantstrainofaspergillusnigergcmc7usingmolassesbasedmedium AT iqbaljaved citricacidfermentationbymutantstrainofaspergillusnigergcmc7usingmolassesbasedmedium |
| _version_ |
1718441703799521280 |