Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida

The purpose of this project was to evaluate the biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida. Gramoxones active ingredient is Paraquat (1,1-dimethyl-4,4-bipyridylium) and Matanchas is 2,4-D (2,4-dichlorophenoxiacetic acid). To carry out...

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Autores principales:	Kopytko,Maria, Chalela,Graciela, Zauscher,Fernando
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200013
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spelling	oai:scielo:S0717-345820020002000132003-08-19Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putidaKopytko,MariaChalela,GracielaZauscher,Fernando The purpose of this project was to evaluate the biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida. Gramoxones active ingredient is Paraquat (1,1-dimethyl-4,4-bipyridylium) and Matanchas is 2,4-D (2,4-dichlorophenoxiacetic acid). To carry out the biodegradation experiments, a factorial design 2³ was executed, where the independent variables were: herbicide concentration, nutrient concentration and the use or no use of activated charcoal (AC) in the same experiment with bacteria. Duplicate experiments were performed for a period of three days using 500 ml bioreactors. The best results obtained when no AC was used were: 47,29% degradation for Gramoxone (by UV/VIS) and 68.72% for Matancha (by HPLC). The treatments carried out with the use of AC for either of the two herbicides gave more than 95% degradation at 72 h. In fact, under these conditions most of the experiments showed at least a 90% removal at 24 h. It is clear that the dual combination of adsorption by AC and biological activity by the bacteria was very effective, in particular when at least 10% nutrients were added. The statistical study of the results showed that AC is a highly significant variable, and that there are significant interactions between the three variables used in the experimental design. The application of these experiments to wastewater (COD = 584.11) confirmed that it is possible to remove more than 90% of either of these herbicides in a 24 h period.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.2 20022002-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200013en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	The purpose of this project was to evaluate the biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida. Gramoxones active ingredient is Paraquat (1,1-dimethyl-4,4-bipyridylium) and Matanchas is 2,4-D (2,4-dichlorophenoxiacetic acid). To carry out the biodegradation experiments, a factorial design 2³ was executed, where the independent variables were: herbicide concentration, nutrient concentration and the use or no use of activated charcoal (AC) in the same experiment with bacteria. Duplicate experiments were performed for a period of three days using 500 ml bioreactors. The best results obtained when no AC was used were: 47,29% degradation for Gramoxone (by UV/VIS) and 68.72% for Matancha (by HPLC). The treatments carried out with the use of AC for either of the two herbicides gave more than 95% degradation at 72 h. In fact, under these conditions most of the experiments showed at least a 90% removal at 24 h. It is clear that the dual combination of adsorption by AC and biological activity by the bacteria was very effective, in particular when at least 10% nutrients were added. The statistical study of the results showed that AC is a highly significant variable, and that there are significant interactions between the three variables used in the experimental design. The application of these experiments to wastewater (COD = 584.11) confirmed that it is possible to remove more than 90% of either of these herbicides in a 24 h period.
author	Kopytko,Maria Chalela,Graciela Zauscher,Fernando
spellingShingle	Kopytko,Maria Chalela,Graciela Zauscher,Fernando Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida
author_facet	Kopytko,Maria Chalela,Graciela Zauscher,Fernando
author_sort	Kopytko,Maria
title	Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida
title_short	Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida
title_full	Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida
title_fullStr	Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida
title_full_unstemmed	Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida
title_sort	biodegradation of two commercial herbicides (gramoxone and matancha) by the bacteria pseudomonas putida
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200013
work_keys_str_mv	AT kopytkomaria biodegradationoftwocommercialherbicidesgramoxoneandmatanchabythebacteriapseudomonasputida AT chalelagraciela biodegradationoftwocommercialherbicidesgramoxoneandmatanchabythebacteriapseudomonasputida AT zauscherfernando biodegradationoftwocommercialherbicidesgramoxoneandmatanchabythebacteriapseudomonasputida
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Biodegradation of two commercial herbicides (Gramoxone and Matancha) by the bacteria Pseudomonas putida

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