Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems

Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein c...

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Autores principales:	Bollati Fogolín,Mariela, Eberhardt,Marcos Oggero, Kratje,Ricardo, Etcheverrigaray,Marina
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300008
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spelling	oai:scielo:S0717-345820020003000082003-08-18Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systemsBollati Fogolín,MarielaEberhardt,Marcos OggeroKratje,RicardoEtcheverrigaray,Marina Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein content, and therefore useful for determining concentrations of the cytokine in production processes. Among these assays, the competitive ELISA, developed with a MAb that recognises an epitope not involved in glycosylation, was the only one suitable to quantify the glycosylated form of rhGM-CSF produced in mammalian cell cultures.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.3 20022002-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300008en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein content, and therefore useful for determining concentrations of the cytokine in production processes. Among these assays, the competitive ELISA, developed with a MAb that recognises an epitope not involved in glycosylation, was the only one suitable to quantify the glycosylated form of rhGM-CSF produced in mammalian cell cultures.
author	Bollati Fogolín,Mariela Eberhardt,Marcos Oggero Kratje,Ricardo Etcheverrigaray,Marina
spellingShingle	Bollati Fogolín,Mariela Eberhardt,Marcos Oggero Kratje,Ricardo Etcheverrigaray,Marina Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
author_facet	Bollati Fogolín,Mariela Eberhardt,Marcos Oggero Kratje,Ricardo Etcheverrigaray,Marina
author_sort	Bollati Fogolín,Mariela
title	Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
title_short	Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
title_full	Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
title_fullStr	Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
title_full_unstemmed	Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems
title_sort	choice of the adequate quantification method for recombinant human gm-csf produced in different host systems
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300008
work_keys_str_mv	AT bollatifogolinmariela choiceoftheadequatequantificationmethodforrecombinanthumangmcsfproducedindifferenthostsystems AT eberhardtmarcosoggero choiceoftheadequatequantificationmethodforrecombinanthumangmcsfproducedindifferenthostsystems AT kratjericardo choiceoftheadequatequantificationmethodforrecombinanthumangmcsfproducedindifferenthostsystems AT etcheverrigaraymarina choiceoftheadequatequantificationmethodforrecombinanthumangmcsfproducedindifferenthostsystems
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Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems

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