Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor

The present investigation deals with the kinetics of submerged citric acid fermentation by Aspergillus niger using blackstrap molasses as the basal fermentation media. A laboratory scale stirred fermentor of 15-L capacity having working volume of 9-L was used for cultivation process and nutritional...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ali,Sikander, Haq,Ikram-ul-, Qadeer,M.A., Iqbal,Javed
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2002
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300010
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582002000300010
record_format	dspace
spelling	oai:scielo:S0717-345820020003000102003-08-18Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentorAli,SikanderHaq,Ikram-ul-Qadeer,M.A.Iqbal,Javed The present investigation deals with the kinetics of submerged citric acid fermentation by Aspergillus niger using blackstrap molasses as the basal fermentation media. A laboratory scale stirred fermentor of 15-L capacity having working volume of 9-L was used for cultivation process and nutritional analysis. Among the 10 stock cultures of Aspergillus niger, the strain GCBT7 was found to enhance citric acid production. This strain was subjected to parametric studies. Major effects were caused due to oxygen tension (1.0 l/l/min), pH value (6.0) and incubation temperature (30ºC). All fermentations were carried out following the growth on 150 g/l raw molasses sugars for 144 hours. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. Ammonium nitrate (0.2%) was added as nitrogen source. Maximum citric acid production (99.56 ± 3.5a g/l) was achieved by Aspergillus niger GCBT7. The dry cell mass and sugar consumption were 18.5 and 96.55 g/l, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity of GCBT7 (qp = 0.074 ± 0.02a g/g cells/h) was several folds higher than other strains. The specific production rate and growth coefficient revealed the hyperproducibility of citric acid using mutant GCBT7.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.3 20022002-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300010en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	The present investigation deals with the kinetics of submerged citric acid fermentation by Aspergillus niger using blackstrap molasses as the basal fermentation media. A laboratory scale stirred fermentor of 15-L capacity having working volume of 9-L was used for cultivation process and nutritional analysis. Among the 10 stock cultures of Aspergillus niger, the strain GCBT7 was found to enhance citric acid production. This strain was subjected to parametric studies. Major effects were caused due to oxygen tension (1.0 l/l/min), pH value (6.0) and incubation temperature (30ºC). All fermentations were carried out following the growth on 150 g/l raw molasses sugars for 144 hours. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. Ammonium nitrate (0.2%) was added as nitrogen source. Maximum citric acid production (99.56 ± 3.5a g/l) was achieved by Aspergillus niger GCBT7. The dry cell mass and sugar consumption were 18.5 and 96.55 g/l, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity of GCBT7 (qp = 0.074 ± 0.02a g/g cells/h) was several folds higher than other strains. The specific production rate and growth coefficient revealed the hyperproducibility of citric acid using mutant GCBT7.
author	Ali,Sikander Haq,Ikram-ul- Qadeer,M.A. Iqbal,Javed
spellingShingle	Ali,Sikander Haq,Ikram-ul- Qadeer,M.A. Iqbal,Javed Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor
author_facet	Ali,Sikander Haq,Ikram-ul- Qadeer,M.A. Iqbal,Javed
author_sort	Ali,Sikander
title	Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor
title_short	Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor
title_full	Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor
title_fullStr	Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor
title_full_unstemmed	Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor
title_sort	production of citric acid by aspergillus niger using cane molasses in a stirred fermentor
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300010
work_keys_str_mv	AT alisikander productionofcitricacidbyaspergillusnigerusingcanemolassesinastirredfermentor AT haqikramul productionofcitricacidbyaspergillusnigerusingcanemolassesinastirredfermentor AT qadeerma productionofcitricacidbyaspergillusnigerusingcanemolassesinastirredfermentor AT iqbaljaved productionofcitricacidbyaspergillusnigerusingcanemolassesinastirredfermentor
_version_	1718441707649892352

Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentor

Ejemplares similares