A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads
The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple,...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2002
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oai:scielo:S0717-345820020003000122003-08-18A laboratory scale device for microencapsulation of genetically engineered cells into alginate beadsLeón Fiszman,GabrielKarara,Armando LuisFinocchiaro,Liliana María ElenaGlikin,Gerardo Claudio The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.5 n.3 20022002-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300012en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
The microencapsulation of recombinant cells, widely used for in vitro high-density cell culture, is a novel and potentially cost-effective method of in vivo heterologous protein delivery, where the protein producing cells are immunologically protected from tissue rejection. We report here a simple, reliable and inexpensive laboratory method to generate calcium alginate microcapsules containing genetically engineered, interleukin-2 expressing, Chinese hamster ovary (CHO) cells. |
| author |
León Fiszman,Gabriel Karara,Armando Luis Finocchiaro,Liliana María Elena Glikin,Gerardo Claudio |
| spellingShingle |
León Fiszman,Gabriel Karara,Armando Luis Finocchiaro,Liliana María Elena Glikin,Gerardo Claudio A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| author_facet |
León Fiszman,Gabriel Karara,Armando Luis Finocchiaro,Liliana María Elena Glikin,Gerardo Claudio |
| author_sort |
León Fiszman,Gabriel |
| title |
A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| title_short |
A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| title_full |
A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| title_fullStr |
A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| title_full_unstemmed |
A laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| title_sort |
laboratory scale device for microencapsulation of genetically engineered cells into alginate beads |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2002 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300012 |
| work_keys_str_mv |
AT leonfiszmangabriel alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads AT kararaarmandoluis alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads AT finocchiarolilianamariaelena alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads AT glikingerardoclaudio alaboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads AT leonfiszmangabriel laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads AT kararaarmandoluis laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads AT finocchiarolilianamariaelena laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads AT glikingerardoclaudio laboratoryscaledeviceformicroencapsulationofgeneticallyengineeredcellsintoalginatebeads |
| _version_ |
1718441708193054720 |