Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta

This paper reports biotransformation of (L)-citronellal to (L)-citronellol using free and immobilized cells of Rhodotorula minuta. The culture preparation variables such as pH, temperature and incubation period for obtaining maximum cell growth of R. minuta were optimized. The optimized culture cond...

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Autores principales:	Velankar,Harshad Ravindra, Heble,Mohan Ram
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2003
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200004
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spelling	oai:scielo:S0717-345820030002000042004-05-13Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minutaVelankar,Harshad RavindraHeble,Mohan Ram This paper reports biotransformation of (L)-citronellal to (L)-citronellol using free and immobilized cells of Rhodotorula minuta. The culture preparation variables such as pH, temperature and incubation period for obtaining maximum cell growth of R. minuta were optimized. The optimized culture conditions for free and immobilized cells of R. minuta. have been compared for (L)-citronellal biotransformation. The various factors such as the optimum substrate concentration and the time of substrate addition at varying cell concentrations during the growth of yeast culture were also studied. Highest (L)-citronellol concentration of 3.5 gl-1 was obtained with free cell catalyzed biotransformation at pH 5.5, 27ºC and 150 rpm after 8 hrs using initial (L)-citronellal concentration of 4.47 gl-1. Alginate immobilized R. minuta cells could optimally biotransform similar substrate concentration to 3.3 gl-1 (L)-citronellol at pH 6, 27ºC and 150 rpm after 8 hrs. Immobilized cells could be reused twice after the first run and the product concentrations of 2.63 gl-1 and 1.52 gl-1 were obtained during the first and the second reuseinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.2 20032003-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200004en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	This paper reports biotransformation of (L)-citronellal to (L)-citronellol using free and immobilized cells of Rhodotorula minuta. The culture preparation variables such as pH, temperature and incubation period for obtaining maximum cell growth of R. minuta were optimized. The optimized culture conditions for free and immobilized cells of R. minuta. have been compared for (L)-citronellal biotransformation. The various factors such as the optimum substrate concentration and the time of substrate addition at varying cell concentrations during the growth of yeast culture were also studied. Highest (L)-citronellol concentration of 3.5 gl-1 was obtained with free cell catalyzed biotransformation at pH 5.5, 27ºC and 150 rpm after 8 hrs using initial (L)-citronellal concentration of 4.47 gl-1. Alginate immobilized R. minuta cells could optimally biotransform similar substrate concentration to 3.3 gl-1 (L)-citronellol at pH 6, 27ºC and 150 rpm after 8 hrs. Immobilized cells could be reused twice after the first run and the product concentrations of 2.63 gl-1 and 1.52 gl-1 were obtained during the first and the second reuse
author	Velankar,Harshad Ravindra Heble,Mohan Ram
spellingShingle	Velankar,Harshad Ravindra Heble,Mohan Ram Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta
author_facet	Velankar,Harshad Ravindra Heble,Mohan Ram
author_sort	Velankar,Harshad Ravindra
title	Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta
title_short	Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta
title_full	Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta
title_fullStr	Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta
title_full_unstemmed	Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta
title_sort	biotransformation of (l)-citronellal to (l)-citronellol by free and immobilized rhodotorula minuta
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200004
work_keys_str_mv	AT velankarharshadravindra biotransformationoflcitronellaltolcitronellolbyfreeandimmobilizedrhodotorulaminuta AT heblemohanram biotransformationoflcitronellaltolcitronellolbyfreeandimmobilizedrhodotorulaminuta
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Biotransformation of (L)-citronellal to (L)-citronellol by free and immobilized Rhodotorula minuta

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