Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon mesh
Free-cell cultures of Trametes versicolor were compared with cultures immobilized on nylon mesh in a 2-litre bioreactor for transformation of pentachlorophenol (PCP) and 2,4-dichlorophenol (2,4-DCP), added at intervals to the liquid culture medium over a period of 816 hrs. Increasing amounts of PCP...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2003
|
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200005 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-34582003000200005 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-345820030002000052004-05-13Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon meshSedarati,Mohammad RKeshavarz,TajalliLeontievsky,Alexey AEvans,Christine S. Free-cell cultures of Trametes versicolor were compared with cultures immobilized on nylon mesh in a 2-litre bioreactor for transformation of pentachlorophenol (PCP) and 2,4-dichlorophenol (2,4-DCP), added at intervals to the liquid culture medium over a period of 816 hrs. Increasing amounts of PCP from 200 ppm to 2000 ppm added batchwise to cultures permitted acclimatization of the fungus to these toxic pollutants. A total addition of 2000 ppm of 2,4-DCP and 3400 ppm PCP were removed from the immobilized cultures with 85% of 2,4-DCP and 70% of PCP transformed by enzymes (laccase and Mn-peroxidase), 5% 2,4-DCP and 28% PCP adsorbed by the biomass and 10% 2,4-DCP and 2% PCP retained in the medium at the termination of the fermentation after 1020 hrs. In contrast free-cell cultures in the same medium with the same addition regime of PCP and 2,4-DCP, transformed 20% 2,4-DCP and 12% PCP by enzyme action, adsorbed 58% 2,4-DCP and 80% PCP by the biomass, and retained 22% 2,4-DCP and 8% PCP in the medium. The use of nylon mesh as an immobilization matrix for removal of PCP and 2,4-DCP facilitates more efficient removal of chlorophenols and can be adapted to scale-up for application of large volumes of chlorophenol-containing aqueous effluents.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.2 20032003-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200005en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
Free-cell cultures of Trametes versicolor were compared with cultures immobilized on nylon mesh in a 2-litre bioreactor for transformation of pentachlorophenol (PCP) and 2,4-dichlorophenol (2,4-DCP), added at intervals to the liquid culture medium over a period of 816 hrs. Increasing amounts of PCP from 200 ppm to 2000 ppm added batchwise to cultures permitted acclimatization of the fungus to these toxic pollutants. A total addition of 2000 ppm of 2,4-DCP and 3400 ppm PCP were removed from the immobilized cultures with 85% of 2,4-DCP and 70% of PCP transformed by enzymes (laccase and Mn-peroxidase), 5% 2,4-DCP and 28% PCP adsorbed by the biomass and 10% 2,4-DCP and 2% PCP retained in the medium at the termination of the fermentation after 1020 hrs. In contrast free-cell cultures in the same medium with the same addition regime of PCP and 2,4-DCP, transformed 20% 2,4-DCP and 12% PCP by enzyme action, adsorbed 58% 2,4-DCP and 80% PCP by the biomass, and retained 22% 2,4-DCP and 8% PCP in the medium. The use of nylon mesh as an immobilization matrix for removal of PCP and 2,4-DCP facilitates more efficient removal of chlorophenols and can be adapted to scale-up for application of large volumes of chlorophenol-containing aqueous effluents. |
| author |
Sedarati,Mohammad R Keshavarz,Tajalli Leontievsky,Alexey A Evans,Christine S. |
| spellingShingle |
Sedarati,Mohammad R Keshavarz,Tajalli Leontievsky,Alexey A Evans,Christine S. Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon mesh |
| author_facet |
Sedarati,Mohammad R Keshavarz,Tajalli Leontievsky,Alexey A Evans,Christine S. |
| author_sort |
Sedarati,Mohammad R |
| title |
Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon mesh |
| title_short |
Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon mesh |
| title_full |
Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon mesh |
| title_fullStr |
Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon mesh |
| title_full_unstemmed |
Transformation of high concentrations of chlorophenols by the white-rot basidiomycete Trametes versicolor immobilized on nylon mesh |
| title_sort |
transformation of high concentrations of chlorophenols by the white-rot basidiomycete trametes versicolor immobilized on nylon mesh |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2003 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200005 |
| work_keys_str_mv |
AT sedaratimohammadr transformationofhighconcentrationsofchlorophenolsbythewhiterotbasidiomycetetrametesversicolorimmobilizedonnylonmesh AT keshavarztajalli transformationofhighconcentrationsofchlorophenolsbythewhiterotbasidiomycetetrametesversicolorimmobilizedonnylonmesh AT leontievskyalexeya transformationofhighconcentrationsofchlorophenolsbythewhiterotbasidiomycetetrametesversicolorimmobilizedonnylonmesh AT evanschristines transformationofhighconcentrationsofchlorophenolsbythewhiterotbasidiomycetetrametesversicolorimmobilizedonnylonmesh |
| _version_ |
1718441712930521088 |