Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility
Albino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from...
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Pontificia Universidad Católica de Valparaíso
2003
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oai:scielo:S0717-345820030002000072004-05-13Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertilityLakrod,KamolnanChaisrisook,ChuleeSkinner,Daniel Z Albino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. Both of the independent transformants formed cleistothecia but ascospore formation was greatly reduced or absent, suggesting the integration and expression of the genes carried on pMOcosX interfered with fertilization and/or ascospore formation in this homothallic fungus.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.2 20032003-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200007en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
Albino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. Both of the independent transformants formed cleistothecia but ascospore formation was greatly reduced or absent, suggesting the integration and expression of the genes carried on pMOcosX interfered with fertilization and/or ascospore formation in this homothallic fungus. |
| author |
Lakrod,Kamolnan Chaisrisook,Chulee Skinner,Daniel Z |
| spellingShingle |
Lakrod,Kamolnan Chaisrisook,Chulee Skinner,Daniel Z Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility |
| author_facet |
Lakrod,Kamolnan Chaisrisook,Chulee Skinner,Daniel Z |
| author_sort |
Lakrod,Kamolnan |
| title |
Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility |
| title_short |
Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility |
| title_full |
Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility |
| title_fullStr |
Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility |
| title_full_unstemmed |
Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility |
| title_sort |
transformation of monascus purpureus to hygromycin b resistance with cosmid pmocosx reduces fertility |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2003 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200007 |
| work_keys_str_mv |
AT lakrodkamolnan transformationofmonascuspurpureustohygromycinbresistancewithcosmidpmocosxreducesfertility AT chaisrisookchulee transformationofmonascuspurpureustohygromycinbresistancewithcosmidpmocosxreducesfertility AT skinnerdanielz transformationofmonascuspurpureustohygromycinbresistancewithcosmidpmocosxreducesfertility |
| _version_ |
1718441713463197696 |