Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology

beta-Xylosidase recovery by reversed micelles using CTAB cationic surfactant was performed under different experimental conditions. A 2²-full orthogonal design with center points was employed to optimize the recovery of this enzyme and to evaluate the influence of the factors CTAB (A) and butanol co...

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Autores principales:	Hasmann,Francislene Andreia, Vieira Cortez,Daniela, Pessoa Júnior,Adalberto, Conceição Roberto,Inês
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2003
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200009
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spelling	oai:scielo:S0717-345820030002000092004-05-13Optimization of beta-xylosidase recovery by reversed micelles using response surface methodologyHasmann,Francislene AndreiaVieira Cortez,DanielaPessoa Júnior,AdalbertoConceição Roberto,Inês beta-Xylosidase recovery by reversed micelles using CTAB cationic surfactant was performed under different experimental conditions. A 2²-full orthogonal design with center points was employed to optimize the recovery of this enzyme and to evaluate the influence of the factors CTAB (A) and butanol concentration (B) on the enzyme extraction. A mathematical model to represent the enzyme recovery as a function of A and B was proposed. According to the model, the CTAB and butanol concentrations necessary to attain the maximum beta-xylosidase recovery (43%) were 0.26 M and 29.4%, respectively. The results showed that the recovery value predicted by the model was similar to that obtained experimentally (44.3%).info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.2 20032003-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200009en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	beta-Xylosidase recovery by reversed micelles using CTAB cationic surfactant was performed under different experimental conditions. A 2²-full orthogonal design with center points was employed to optimize the recovery of this enzyme and to evaluate the influence of the factors CTAB (A) and butanol concentration (B) on the enzyme extraction. A mathematical model to represent the enzyme recovery as a function of A and B was proposed. According to the model, the CTAB and butanol concentrations necessary to attain the maximum beta-xylosidase recovery (43%) were 0.26 M and 29.4%, respectively. The results showed that the recovery value predicted by the model was similar to that obtained experimentally (44.3%).
author	Hasmann,Francislene Andreia Vieira Cortez,Daniela Pessoa Júnior,Adalberto Conceição Roberto,Inês
spellingShingle	Hasmann,Francislene Andreia Vieira Cortez,Daniela Pessoa Júnior,Adalberto Conceição Roberto,Inês Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology
author_facet	Hasmann,Francislene Andreia Vieira Cortez,Daniela Pessoa Júnior,Adalberto Conceição Roberto,Inês
author_sort	Hasmann,Francislene Andreia
title	Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology
title_short	Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology
title_full	Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology
title_fullStr	Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology
title_full_unstemmed	Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology
title_sort	optimization of beta-xylosidase recovery by reversed micelles using response surface methodology
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200009
work_keys_str_mv	AT hasmannfrancisleneandreia optimizationofbetaxylosidaserecoverybyreversedmicellesusingresponsesurfacemethodology AT vieiracortezdaniela optimizationofbetaxylosidaserecoverybyreversedmicellesusingresponsesurfacemethodology AT pessoajunioradalberto optimizationofbetaxylosidaserecoverybyreversedmicellesusingresponsesurfacemethodology AT conceicaorobertoines optimizationofbetaxylosidaserecoverybyreversedmicellesusingresponsesurfacemethodology
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Optimization of beta-xylosidase recovery by reversed micelles using response surface methodology

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