Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia
Abstract <A HREF="../../../../fbpe/img/ejb/v6n3/a02/bip/bip.html">Reprint (BIP)</A> <A HREF="../../../../fbpe/img/ejb/v6n3/a02/reprint.html">Reprint (PDF)</A> Abstract <A HREF="#article">Article </A> <A HREF="#references"...
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Pontificia Universidad Católica de Valparaíso
2003
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oai:scielo:S0717-345820030003000062004-05-04Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemiaOjalvo,Ariana G.Seralena,AlinaVázquez,RaysaMontequín,José FVispo,Nelson S.Silva,RicardoAldama,AlfredoPuchades,YaquelinSorell,Luis T.Lopez-Saura,PedroAlfonso,María A.Simón,RafaelAlí,AlfonsoSeuc,ArmandoHerrera,Luis Abstract <A HREF="../../../../fbpe/img/ejb/v6n3/a02/bip/bip.html">Reprint (BIP)</A> <A HREF="../../../../fbpe/img/ejb/v6n3/a02/reprint.html">Reprint (PDF)</A> Abstract <A HREF="#article">Article </A> <A HREF="#references">References </A> Vascular endothelial growth factor (VEGF), an endothelial cell-specific mitogen, has been shown to promote therapeutic angiogenesis in animal models of critical limb ischemia. Ischemic skeletal muscle is advantageous for taking up and expressing foreign genes transferred as naked plasmid DNA. Accordingly, we investigated the hypothesis that intramuscular administration of naked plasmid DNA encoding the 121-amino acid isoform of VEGF could augment collateral development and tissue perfusion in a dog hindlimb ischemia model. Unilateral hindlimb ischemia was surgically induced in Beagle dogs. Ten days later, animals received intramuscular injections of pVEGF121 plasmid directly in the ischemic muscles. Angiogenic effects were evaluated by angiography, calf blood pressure ratio and vasomotor reserve analyses. Thirty days after gene transfer, angiographically recognizable collateral vessels were increased in pVEGF121-treated animals compared with controls. Improvement in perfusion to the ischemic limb was documented by a significantly higher calf blood pressure ratio for pVEGF121 (0.79 ± 0.05) versus controls (0.56 ± 0.14, P<0.01). Vasomotor reserve assay suggested amelioration in blood availability at the microcirculation level in pVEGF121-treated animals. Hematological variables showed no significant modification due to the treatment. Our results suggest that intramuscular gene transfer of VEGF121 may promote therapeutic angiogenesis in critical limb vascular insufficiency.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.3 20032003-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300006en |
| institution |
Scielo Chile |
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Scielo Chile |
| language |
English |
| description |
Abstract <A HREF="../../../../fbpe/img/ejb/v6n3/a02/bip/bip.html">Reprint (BIP)</A> <A HREF="../../../../fbpe/img/ejb/v6n3/a02/reprint.html">Reprint (PDF)</A> Abstract <A HREF="#article">Article </A> <A HREF="#references">References </A> Vascular endothelial growth factor (VEGF), an endothelial cell-specific mitogen, has been shown to promote therapeutic angiogenesis in animal models of critical limb ischemia. Ischemic skeletal muscle is advantageous for taking up and expressing foreign genes transferred as naked plasmid DNA. Accordingly, we investigated the hypothesis that intramuscular administration of naked plasmid DNA encoding the 121-amino acid isoform of VEGF could augment collateral development and tissue perfusion in a dog hindlimb ischemia model. Unilateral hindlimb ischemia was surgically induced in Beagle dogs. Ten days later, animals received intramuscular injections of pVEGF121 plasmid directly in the ischemic muscles. Angiogenic effects were evaluated by angiography, calf blood pressure ratio and vasomotor reserve analyses. Thirty days after gene transfer, angiographically recognizable collateral vessels were increased in pVEGF121-treated animals compared with controls. Improvement in perfusion to the ischemic limb was documented by a significantly higher calf blood pressure ratio for pVEGF121 (0.79 ± 0.05) versus controls (0.56 ± 0.14, P<0.01). Vasomotor reserve assay suggested amelioration in blood availability at the microcirculation level in pVEGF121-treated animals. Hematological variables showed no significant modification due to the treatment. Our results suggest that intramuscular gene transfer of VEGF121 may promote therapeutic angiogenesis in critical limb vascular insufficiency. |
| author |
Ojalvo,Ariana G. Seralena,Alina Vázquez,Raysa Montequín,José F Vispo,Nelson S. Silva,Ricardo Aldama,Alfredo Puchades,Yaquelin Sorell,Luis T. Lopez-Saura,Pedro Alfonso,María A. Simón,Rafael Alí,Alfonso Seuc,Armando Herrera,Luis |
| spellingShingle |
Ojalvo,Ariana G. Seralena,Alina Vázquez,Raysa Montequín,José F Vispo,Nelson S. Silva,Ricardo Aldama,Alfredo Puchades,Yaquelin Sorell,Luis T. Lopez-Saura,Pedro Alfonso,María A. Simón,Rafael Alí,Alfonso Seuc,Armando Herrera,Luis Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia |
| author_facet |
Ojalvo,Ariana G. Seralena,Alina Vázquez,Raysa Montequín,José F Vispo,Nelson S. Silva,Ricardo Aldama,Alfredo Puchades,Yaquelin Sorell,Luis T. Lopez-Saura,Pedro Alfonso,María A. Simón,Rafael Alí,Alfonso Seuc,Armando Herrera,Luis |
| author_sort |
Ojalvo,Ariana G. |
| title |
Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia |
| title_short |
Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia |
| title_full |
Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia |
| title_fullStr |
Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia |
| title_full_unstemmed |
Therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia |
| title_sort |
therapeutic angiogenesis following intramuscular gene transfer of vascular endothelialgrowth factor 121 in a dog model of hindlimb ischemia |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2003 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300006 |
| work_keys_str_mv |
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| _version_ |
1718441716384530432 |