Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide

A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 m...

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Autores principales: Hernández García,Armando, Pimentel Vázquez,Eulogio, Mena Campos,Jesús
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2003
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300007
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spelling oai:scielo:S0717-345820030003000072004-05-07Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfideHernández García,ArmandoPimentel Vázquez,EulogioMena Campos,Jesús A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 µmol. The developed system was applied using Tsukamurella paurometabola DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 µmol in 24 hrs.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.3 20032003-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300007en
institution Scielo Chile
collection Scielo Chile
language English
description A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 µmol. The developed system was applied using Tsukamurella paurometabola DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 µmol in 24 hrs.
author Hernández García,Armando
Pimentel Vázquez,Eulogio
Mena Campos,Jesús
spellingShingle Hernández García,Armando
Pimentel Vázquez,Eulogio
Mena Campos,Jesús
Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
author_facet Hernández García,Armando
Pimentel Vázquez,Eulogio
Mena Campos,Jesús
author_sort Hernández García,Armando
title Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
title_short Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
title_full Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
title_fullStr Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
title_full_unstemmed Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
title_sort minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2003
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300007
work_keys_str_mv AT hernandezgarciaarmando minibioreactorgascollectorfordeterminingbacteriaproducedhydrogensulfide
AT pimentelvazquezeulogio minibioreactorgascollectorfordeterminingbacteriaproducedhydrogensulfide
AT menacamposjesus minibioreactorgascollectorfordeterminingbacteriaproducedhydrogensulfide
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