Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide
A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 m...
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Pontificia Universidad Católica de Valparaíso
2003
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oai:scielo:S0717-345820030003000072004-05-07Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfideHernández García,ArmandoPimentel Vázquez,EulogioMena Campos,Jesús A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 µmol. The developed system was applied using Tsukamurella paurometabola DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 µmol in 24 hrs.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.3 20032003-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300007en |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
English |
description |
A simple and economical minibioreactor-gas collector system for determination of hydrogen sulfide produced by a microorganism was designed. The detection of hydrogen sulfide was based on the reaction between the hydrogen sulfide in the gas stream from the culture, and a lead acetate solution 0.090 mol/L, contained in a tube gas collector; the conductimetric back titration of lead excess was made, and hydrogen sulfide was quantified indirectly, being the detection limit 0.5 µmol. The developed system was applied using Tsukamurella paurometabola DSM 20162 as a model, being the amount of hydrogen sulfide produced, 1.2 µmol in 24 hrs. |
author |
Hernández García,Armando Pimentel Vázquez,Eulogio Mena Campos,Jesús |
spellingShingle |
Hernández García,Armando Pimentel Vázquez,Eulogio Mena Campos,Jesús Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide |
author_facet |
Hernández García,Armando Pimentel Vázquez,Eulogio Mena Campos,Jesús |
author_sort |
Hernández García,Armando |
title |
Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide |
title_short |
Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide |
title_full |
Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide |
title_fullStr |
Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide |
title_full_unstemmed |
Minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide |
title_sort |
minibioreactor-gas collector for determining bacteria-produced hydrogen sulfide |
publisher |
Pontificia Universidad Católica de Valparaíso |
publishDate |
2003 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300007 |
work_keys_str_mv |
AT hernandezgarciaarmando minibioreactorgascollectorfordeterminingbacteriaproducedhydrogensulfide AT pimentelvazquezeulogio minibioreactorgascollectorfordeterminingbacteriaproducedhydrogensulfide AT menacamposjesus minibioreactorgascollectorfordeterminingbacteriaproducedhydrogensulfide |
_version_ |
1718441716681277440 |