Root induction in radiata pine using Agrobacterium rhizogenes

Root induction using Agrobacterium rhizogenes was conducted in hypocotyl explants, intact seedlings, de-rooted seedling cuttings and adventitious shoots of radiata pine (Pinus radiata D. Don). Use of two A. rhizogenes strains (A4T and LB9402), with or without application of IBA, can trigger root for...

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Autores principales: Li,Mingshan, Leung,David W.M.
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2003
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300010
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spelling oai:scielo:S0717-345820030003000102004-05-04Root induction in radiata pine using Agrobacterium rhizogenesLi,MingshanLeung,David W.M. Root induction using Agrobacterium rhizogenes was conducted in hypocotyl explants, intact seedlings, de-rooted seedling cuttings and adventitious shoots of radiata pine (Pinus radiata D. Don). Use of two A. rhizogenes strains (A4T and LB9402), with or without application of IBA, can trigger root formation in different explants. Strain LBA9402 was more effective than A4T in increasing rooting percentage and root number. Addition of 4.4 uMIBA to the medium further enhanced rooting from the cultured hypocotyl segments inoculated with the two A. rhizogenes strains. Strain LBA9402+IBA induced in about 75% of the cultured hypocotyl segments to form roots. In contrast the controls failed to initiate roots on intact seedlings or cultured segments in presence or absence of IBA. Rooting of adventitious shoots from 3 year-old radiata pine was improved following inoculation with LBA9402, suggesting that this rooting treatment has potential to aid clonal propagation of radiata pine.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.6 n.3 20032003-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300010en
institution Scielo Chile
collection Scielo Chile
language English
description Root induction using Agrobacterium rhizogenes was conducted in hypocotyl explants, intact seedlings, de-rooted seedling cuttings and adventitious shoots of radiata pine (Pinus radiata D. Don). Use of two A. rhizogenes strains (A4T and LB9402), with or without application of IBA, can trigger root formation in different explants. Strain LBA9402 was more effective than A4T in increasing rooting percentage and root number. Addition of 4.4 uMIBA to the medium further enhanced rooting from the cultured hypocotyl segments inoculated with the two A. rhizogenes strains. Strain LBA9402+IBA induced in about 75% of the cultured hypocotyl segments to form roots. In contrast the controls failed to initiate roots on intact seedlings or cultured segments in presence or absence of IBA. Rooting of adventitious shoots from 3 year-old radiata pine was improved following inoculation with LBA9402, suggesting that this rooting treatment has potential to aid clonal propagation of radiata pine.
author Li,Mingshan
Leung,David W.M.
spellingShingle Li,Mingshan
Leung,David W.M.
Root induction in radiata pine using Agrobacterium rhizogenes
author_facet Li,Mingshan
Leung,David W.M.
author_sort Li,Mingshan
title Root induction in radiata pine using Agrobacterium rhizogenes
title_short Root induction in radiata pine using Agrobacterium rhizogenes
title_full Root induction in radiata pine using Agrobacterium rhizogenes
title_fullStr Root induction in radiata pine using Agrobacterium rhizogenes
title_full_unstemmed Root induction in radiata pine using Agrobacterium rhizogenes
title_sort root induction in radiata pine using agrobacterium rhizogenes
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2003
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000300010
work_keys_str_mv AT limingshan rootinductioninradiatapineusingagrobacteriumrhizogenes
AT leungdavidwm rootinductioninradiatapineusingagrobacteriumrhizogenes
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