Quantitative real-time PCR method to detect changes in specific transcript and total RNA amounts
Quantitative real-time PCR (qRT-PCR), used in conjunction with reverse transcriptase, has been applied to the determination of the number of copies of a transcript per unit mass of RNA, but did not indicate any change in the amount of total RNA per mass of tissue. In the present work, we described a...
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| Autores principales: | , |
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2004
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000100008 |
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