Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acid
Lactobacillus rhamnosus, a facultative anaerobe, which produces L (+) lactic acid and ethanol under anaerobic conditions, is used in the present study. An adh- mutant of Lactobacillus rhamnosus MTCC 1408, was developed by chemical mutagenesis, which could produce pure L(+) lactic acid as the only pr...
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Pontificia Universidad Católica de Valparaíso
2004
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oai:scielo:S0717-345820040001000102004-09-09Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acidNarayanan,NijuRoychoudhury,Pradip K.Srivastava,Aradhana Lactobacillus rhamnosus, a facultative anaerobe, which produces L (+) lactic acid and ethanol under anaerobic conditions, is used in the present study. An adh- mutant of Lactobacillus rhamnosus MTCC 1408, was developed by chemical mutagenesis, which could produce pure L(+) lactic acid as the only product. Batch fermentation kinetics of the wild type and the mutant strain were studied in glucose-yeast extract medium under conditions of temperature 40ºC and pH 6.2 anaerobically. The biomass yield was similar in both wild type and mutant strains, however lactic acid yield increased by 6.6%. A chemically defined media was optimized for supplementation of succinate, acetate and citrate for better biomass formation using single variable optimisation. It was further optimised for varying concentrations of vitamins, amino acids and trace metals by response surface method. The batch biomass yield (0.1g/g) and lactic acid yield (0.88g/g) in the optimised chemically defined media were similar to those obtained in the glucose-yeast extract medium.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.1 20042004-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000100010en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
Lactobacillus rhamnosus, a facultative anaerobe, which produces L (+) lactic acid and ethanol under anaerobic conditions, is used in the present study. An adh- mutant of Lactobacillus rhamnosus MTCC 1408, was developed by chemical mutagenesis, which could produce pure L(+) lactic acid as the only product. Batch fermentation kinetics of the wild type and the mutant strain were studied in glucose-yeast extract medium under conditions of temperature 40ºC and pH 6.2 anaerobically. The biomass yield was similar in both wild type and mutant strains, however lactic acid yield increased by 6.6%. A chemically defined media was optimized for supplementation of succinate, acetate and citrate for better biomass formation using single variable optimisation. It was further optimised for varying concentrations of vitamins, amino acids and trace metals by response surface method. The batch biomass yield (0.1g/g) and lactic acid yield (0.88g/g) in the optimised chemically defined media were similar to those obtained in the glucose-yeast extract medium. |
| author |
Narayanan,Niju Roychoudhury,Pradip K. Srivastava,Aradhana |
| spellingShingle |
Narayanan,Niju Roychoudhury,Pradip K. Srivastava,Aradhana Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acid |
| author_facet |
Narayanan,Niju Roychoudhury,Pradip K. Srivastava,Aradhana |
| author_sort |
Narayanan,Niju |
| title |
Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acid |
| title_short |
Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acid |
| title_full |
Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acid |
| title_fullStr |
Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acid |
| title_full_unstemmed |
Isolation of adh mutant of Lactobacillus rhamnosus for production of L(+) Lactic acid |
| title_sort |
isolation of adh mutant of lactobacillus rhamnosus for production of l(+) lactic acid |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2004 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000100010 |
| work_keys_str_mv |
AT narayananniju isolationofadhmutantoflactobacillusrhamnosusforproductionofllacticacid AT roychoudhurypradipk isolationofadhmutantoflactobacillusrhamnosusforproductionofllacticacid AT srivastavaaradhana isolationofadhmutantoflactobacillusrhamnosusforproductionofllacticacid |
| _version_ |
1718441720779112448 |