Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast

The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucro...

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Autores principales:	Trujillo Toledo,Luis Enrique, Gómez Riera,Raúl, Banguela Castillo,Alexander, Soto Romero,Melvis, Arrieta Sosa,Juan Gabriel, Hernández García,Lázaro
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2004
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200005
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spelling	oai:scielo:S0717-345820040002000052004-11-03Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeastTrujillo Toledo,Luis EnriqueGómez Riera,RaúlBanguela Castillo,AlexanderSoto Romero,MelvisArrieta Sosa,Juan GabrielHernández García,Lázaro The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixturesinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.2 20042004-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200005en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures
author	Trujillo Toledo,Luis Enrique Gómez Riera,Raúl Banguela Castillo,Alexander Soto Romero,Melvis Arrieta Sosa,Juan Gabriel Hernández García,Lázaro
spellingShingle	Trujillo Toledo,Luis Enrique Gómez Riera,Raúl Banguela Castillo,Alexander Soto Romero,Melvis Arrieta Sosa,Juan Gabriel Hernández García,Lázaro Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
author_facet	Trujillo Toledo,Luis Enrique Gómez Riera,Raúl Banguela Castillo,Alexander Soto Romero,Melvis Arrieta Sosa,Juan Gabriel Hernández García,Lázaro
author_sort	Trujillo Toledo,Luis Enrique
title	Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
title_short	Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
title_full	Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
title_fullStr	Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
title_full_unstemmed	Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
title_sort	catalytical properties of n-glycosylated gluconacetobacter diazotrophicus levansucrase produced in yeast
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200005
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Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast

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