Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression
The technology of tetracycline-inducible gene expression has been successfully used in experimental biology to identify the function and downstream signaling pathways of an interested gene. It has been significantly improved to meet the criteria with specificity to exogenous non-toxic inducers, inde...
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Pontificia Universidad Católica de Valparaíso
2004
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oai:scielo:S0717-345820040002000132004-11-03Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expressionSong,PingWang,Hwa-Chain Robert The technology of tetracycline-inducible gene expression has been successfully used in experimental biology to identify the function and downstream signaling pathways of an interested gene. It has been significantly improved to meet the criteria with specificity to exogenous non-toxic inducers, independent regulation from cellular pathways, and dose-dependent inducibility and reversibility. However, to establish tetracycline-inducible gene expression in mammalian cells is still a time-and effort-consuming process. With a tetracycline-inducible gene expression system T-REx, we have developed a practical protocol to use the oncogenic H-ras gene as a dominant reporter gene to increase efficiency in attaining desired cell lines in which ectopic expression of a particular gene in cells can be introduced and reversibly induced by the presence or absence of tetracycline in cultures.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.2 20042004-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200013en |
| institution |
Scielo Chile |
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Scielo Chile |
| language |
English |
| description |
The technology of tetracycline-inducible gene expression has been successfully used in experimental biology to identify the function and downstream signaling pathways of an interested gene. It has been significantly improved to meet the criteria with specificity to exogenous non-toxic inducers, independent regulation from cellular pathways, and dose-dependent inducibility and reversibility. However, to establish tetracycline-inducible gene expression in mammalian cells is still a time-and effort-consuming process. With a tetracycline-inducible gene expression system T-REx, we have developed a practical protocol to use the oncogenic H-ras gene as a dominant reporter gene to increase efficiency in attaining desired cell lines in which ectopic expression of a particular gene in cells can be introduced and reversibly induced by the presence or absence of tetracycline in cultures. |
| author |
Song,Ping Wang,Hwa-Chain Robert |
| spellingShingle |
Song,Ping Wang,Hwa-Chain Robert Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression |
| author_facet |
Song,Ping Wang,Hwa-Chain Robert |
| author_sort |
Song,Ping |
| title |
Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression |
| title_short |
Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression |
| title_full |
Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression |
| title_fullStr |
Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression |
| title_full_unstemmed |
Efficient identification of tetR-expressing cell lines for tetracycline-regulated gene expression |
| title_sort |
efficient identification of tetr-expressing cell lines for tetracycline-regulated gene expression |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2004 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000200013 |
| work_keys_str_mv |
AT songping efficientidentificationoftetrexpressingcelllinesfortetracyclineregulatedgeneexpression AT wanghwachainrobert efficientidentificationoftetrexpressingcelllinesfortetracyclineregulatedgeneexpression |
| _version_ |
1718441723864023040 |