Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena

The influence of carbon and nitrogen sources on the production of exo-glucanase was investigated. The enzyme production was variable according to the carbon or nitrogen source used. Levels of beta-cellobiohydrolase (CBH) were minimal in the presence of even low concentrations of glucose. Enzyme prod...

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Autor principal: Rajoka,Muhammad Ibrahim
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2004
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300007
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spelling oai:scielo:S0717-345820040003000072005-05-31Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigenaRajoka,Muhammad Ibrahim The influence of carbon and nitrogen sources on the production of exo-glucanase was investigated. The enzyme production was variable according to the carbon or nitrogen source used. Levels of beta-cellobiohydrolase (CBH) were minimal in the presence of even low concentrations of glucose. Enzyme production was stimulated by other carbohydrates and thus is subject to carbon source control by easily metabolizable sugars. In Dubos medium, on cellobiose, the cellobiohydrolase titres were 2-to 110-fold higher with cells growing on monomeric sugars and 2.7 times higher than cells growing on other disaccharides. alpha-Cellulose was the most effective inducer of beta-cellobiohydrlase and filter paperase (FPase) activities, followed by kallar grass straw. Exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of Cellulomonas flavigena. Nitrates were the best nitrogen sources and supported greater cell mass, cellobiohydrolase and FPase production. During growth on alpha-cellulose containing 8-fold sodium nitrate concentration, maximum volumetric productivities (Qp) of beta-cellobiohydrolase and FPase were 87.5 and 79.5 IU/l./h respectively and are significantly higher than the values reported for some other potent fungi and bacteria.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.3 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300007en
institution Scielo Chile
collection Scielo Chile
language English
description The influence of carbon and nitrogen sources on the production of exo-glucanase was investigated. The enzyme production was variable according to the carbon or nitrogen source used. Levels of beta-cellobiohydrolase (CBH) were minimal in the presence of even low concentrations of glucose. Enzyme production was stimulated by other carbohydrates and thus is subject to carbon source control by easily metabolizable sugars. In Dubos medium, on cellobiose, the cellobiohydrolase titres were 2-to 110-fold higher with cells growing on monomeric sugars and 2.7 times higher than cells growing on other disaccharides. alpha-Cellulose was the most effective inducer of beta-cellobiohydrlase and filter paperase (FPase) activities, followed by kallar grass straw. Exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of Cellulomonas flavigena. Nitrates were the best nitrogen sources and supported greater cell mass, cellobiohydrolase and FPase production. During growth on alpha-cellulose containing 8-fold sodium nitrate concentration, maximum volumetric productivities (Qp) of beta-cellobiohydrolase and FPase were 87.5 and 79.5 IU/l./h respectively and are significantly higher than the values reported for some other potent fungi and bacteria.
author Rajoka,Muhammad Ibrahim
spellingShingle Rajoka,Muhammad Ibrahim
Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena
author_facet Rajoka,Muhammad Ibrahim
author_sort Rajoka,Muhammad Ibrahim
title Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena
title_short Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena
title_full Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena
title_fullStr Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena
title_full_unstemmed Influence of various fermentation variables on exo-glucanase production in Cellulomonas flavigena
title_sort influence of various fermentation variables on exo-glucanase production in cellulomonas flavigena
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2004
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300007
work_keys_str_mv AT rajokamuhammadibrahim influenceofvariousfermentationvariablesonexoglucanaseproductionincellulomonasflavigena
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