Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart
Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that is angiogenic in vitro and in vivo. Several studies report on gene transfer of VEGF121 to promote angiogenesis in the ischemic myocardium of animals and patients. We hypothesized that intramyocardial administratio...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2004
|
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300008 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-34582004000300008 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-345820040003000082005-12-12Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heartOjalvo,Ariana GSeralena,AlinaVispo,Nelson SSilva,RicardoGonzalez,NoelGuevara,LuisBatista,Juan FMontequin,Jose F.Chaos,NicolasGonzález,RafaelReima,CamiloPeña,YamileCoca,MarcosPerera,AlejandroVazquez,RaysaPuchades,YaquelinGarcia-Osuna,TamaraDominguez,HebertoReyes,Jose L.Ali,AlfonsoHerrera,Luis Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that is angiogenic in vitro and in vivo. Several studies report on gene transfer of VEGF121 to promote angiogenesis in the ischemic myocardium of animals and patients. We hypothesized that intramyocardial administration of naked plasmid DNA encoding VEGF121 could improve myocardial perfusion and function in a porcine model of myocardial ischemia. Yorkshire swine underwent thoracotomy and placement of an ameroid constrictor on the circumflex coronary artery. Four weeks later, pVEGF121 plasmid was administered into the ischemic myocardium. Four weeks after gene transfer, SPECT imaging demonstrated significant reduction in the ischemic area in pVEGF121-treated animals compared with controls. In the pVEGF121 group, most of the animals evolved from light ischemia to a normal perfusion. In contrast, control animals exhibited similar or impaired ischemic conditions. Our results indicate that intramyocardial gene transfer of VEGF121 as naked plasmid DNA results in significant improvement in myocardial perfusion and function.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.3 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300008en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that is angiogenic in vitro and in vivo. Several studies report on gene transfer of VEGF121 to promote angiogenesis in the ischemic myocardium of animals and patients. We hypothesized that intramyocardial administration of naked plasmid DNA encoding VEGF121 could improve myocardial perfusion and function in a porcine model of myocardial ischemia. Yorkshire swine underwent thoracotomy and placement of an ameroid constrictor on the circumflex coronary artery. Four weeks later, pVEGF121 plasmid was administered into the ischemic myocardium. Four weeks after gene transfer, SPECT imaging demonstrated significant reduction in the ischemic area in pVEGF121-treated animals compared with controls. In the pVEGF121 group, most of the animals evolved from light ischemia to a normal perfusion. In contrast, control animals exhibited similar or impaired ischemic conditions. Our results indicate that intramyocardial gene transfer of VEGF121 as naked plasmid DNA results in significant improvement in myocardial perfusion and function. |
| author |
Ojalvo,Ariana G Seralena,Alina Vispo,Nelson S Silva,Ricardo Gonzalez,Noel Guevara,Luis Batista,Juan F Montequin,Jose F. Chaos,Nicolas González,Rafael Reima,Camilo Peña,Yamile Coca,Marcos Perera,Alejandro Vazquez,Raysa Puchades,Yaquelin Garcia-Osuna,Tamara Dominguez,Heberto Reyes,Jose L. Ali,Alfonso Herrera,Luis |
| spellingShingle |
Ojalvo,Ariana G Seralena,Alina Vispo,Nelson S Silva,Ricardo Gonzalez,Noel Guevara,Luis Batista,Juan F Montequin,Jose F. Chaos,Nicolas González,Rafael Reima,Camilo Peña,Yamile Coca,Marcos Perera,Alejandro Vazquez,Raysa Puchades,Yaquelin Garcia-Osuna,Tamara Dominguez,Heberto Reyes,Jose L. Ali,Alfonso Herrera,Luis Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart |
| author_facet |
Ojalvo,Ariana G Seralena,Alina Vispo,Nelson S Silva,Ricardo Gonzalez,Noel Guevara,Luis Batista,Juan F Montequin,Jose F. Chaos,Nicolas González,Rafael Reima,Camilo Peña,Yamile Coca,Marcos Perera,Alejandro Vazquez,Raysa Puchades,Yaquelin Garcia-Osuna,Tamara Dominguez,Heberto Reyes,Jose L. Ali,Alfonso Herrera,Luis |
| author_sort |
Ojalvo,Ariana G |
| title |
Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart |
| title_short |
Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart |
| title_full |
Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart |
| title_fullStr |
Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart |
| title_full_unstemmed |
Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart |
| title_sort |
intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2004 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300008 |
| work_keys_str_mv |
AT ojalvoarianag intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT seralenaalina intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT visponelsons intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT silvaricardo intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT gonzaleznoel intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT guevaraluis intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT batistajuanf intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT montequinjosef intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT chaosnicolas intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT gonzalezrafael intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT reimacamilo intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT penayamile intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT cocamarcos intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT pereraalejandro intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT vazquezraysa intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT puchadesyaquelin intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT garciaosunatamara intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT dominguezheberto intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT reyesjosel intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT alialfonso intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart AT herreraluis intramyocardialgenetransferofvascularendothelialgrowthfactor121improvesmyocardialperfusionandfunctionintheischemicporcineheart |
| _version_ |
1718441726025138176 |