Plant regeneration of Anthurium andreanum cv Rubrun

Plant regeneration of Anthurium andreanum cv Rubrun

To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 muM BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium conta...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Vargas,Teresa E., Mejías,Alexander, Oropeza,Maira, de García,Eva
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2004
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300010
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0717-34582004000300010
record_format dspace
spelling oai:scielo:S0717-345820040003000102005-05-31Plant regeneration of Anthurium andreanum cv RubrunVargas,Teresa E.Mejías,AlexanderOropeza,Mairade García,Eva To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 muM BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 muM BA and 0.05 muM NAA. On average, 3.6 shoots per explant were obtained. Four weeks old in vitro plants from germinated seeds and the plantlets obtained from micro-cuttings, showed callus proliferation at the stem base. These tissues were subcultured on a medium supplemented with 8.9 muM BA and 2.7 muM NAA. After 6 weeks of culture, about 43.8 plantlets per square cm of callus were obtained. Anatomical studies showed the organogenic nature of these calli. Anthurium andreanum plants regenerated by organogenesis were transferred to pots and a rate of 80% of plant acclimatization was obtained.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.3 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300010en
institution Scielo Chile
collection Scielo Chile
language English
description To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 muM BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 muM BA and 0.05 muM NAA. On average, 3.6 shoots per explant were obtained. Four weeks old in vitro plants from germinated seeds and the plantlets obtained from micro-cuttings, showed callus proliferation at the stem base. These tissues were subcultured on a medium supplemented with 8.9 muM BA and 2.7 muM NAA. After 6 weeks of culture, about 43.8 plantlets per square cm of callus were obtained. Anatomical studies showed the organogenic nature of these calli. Anthurium andreanum plants regenerated by organogenesis were transferred to pots and a rate of 80% of plant acclimatization was obtained.
author Vargas,Teresa E.
Mejías,Alexander
Oropeza,Maira
de García,Eva
spellingShingle Vargas,Teresa E.
Mejías,Alexander
Oropeza,Maira
de García,Eva
Plant regeneration of Anthurium andreanum cv Rubrun
author_facet Vargas,Teresa E.
Mejías,Alexander
Oropeza,Maira
de García,Eva
author_sort Vargas,Teresa E.
title Plant regeneration of Anthurium andreanum cv Rubrun
title_short Plant regeneration of Anthurium andreanum cv Rubrun
title_full Plant regeneration of Anthurium andreanum cv Rubrun
title_fullStr Plant regeneration of Anthurium andreanum cv Rubrun
title_full_unstemmed Plant regeneration of Anthurium andreanum cv Rubrun
title_sort plant regeneration of anthurium andreanum cv rubrun
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2004
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300010
work_keys_str_mv AT vargasteresae plantregenerationofanthuriumandreanumcvrubrun
AT mejiasalexander plantregenerationofanthuriumandreanumcvrubrun
AT oropezamaira plantregenerationofanthuriumandreanumcvrubrun
AT degarciaeva plantregenerationofanthuriumandreanumcvrubrun
_version_ 1718441726493851648

Ejemplares similares