Preliminary results of using ESR to examine biofilms

This preliminary work shows ESR (Electron Spin Resonance) can be used to detect biofilms, particularly from Fe-metabolising bacteria. A film was detected by ESR as early as 1 day, hence possibly more sensitively than by fluorescent methods. Films can probably be detected as early as one hour. Spectr...

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Autores principales:	Whitehead,Neil Evan, Tani,Atushi, Kazue,Tazaki, Motoji,Ikeya
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2004
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300011
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spelling	oai:scielo:S0717-345820040003000112005-05-31Preliminary results of using ESR to examine biofilmsWhitehead,Neil EvanTani,AtushiKazue,TazakiMotoji,Ikeya This preliminary work shows ESR (Electron Spin Resonance) can be used to detect biofilms, particularly from Fe-metabolising bacteria. A film was detected by ESR as early as 1 day, hence possibly more sensitively than by fluorescent methods. Films can probably be detected as early as one hour. Spectra contain a very broad peak at g=2.13, probably due to ferrihydrite. Results of field experiments from streams and ponds in New Zealand and Japan, particularly the Minoh River, showed a general increase of ferrihydrite with time. Loss by exfoliation was later than 20 days. The rate of accumulation was faster in a nutrient-rich stagnant pond. Hematite (g=4.3) was often observed, magnetite (g=9) once, and usually small amounts of a common bacterial decay product. The latter was detected for at least 18 months film storage. ESR is a particularly good tool for observing the growth of oxic biofilms containing Fe-metabolising bacteria, and should be just as sensitive for observing Mn-metabolising bacteria in reducing conditions.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.3 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300011en
institution	Scielo Chile
collection	Scielo Chile
language	English
description	This preliminary work shows ESR (Electron Spin Resonance) can be used to detect biofilms, particularly from Fe-metabolising bacteria. A film was detected by ESR as early as 1 day, hence possibly more sensitively than by fluorescent methods. Films can probably be detected as early as one hour. Spectra contain a very broad peak at g=2.13, probably due to ferrihydrite. Results of field experiments from streams and ponds in New Zealand and Japan, particularly the Minoh River, showed a general increase of ferrihydrite with time. Loss by exfoliation was later than 20 days. The rate of accumulation was faster in a nutrient-rich stagnant pond. Hematite (g=4.3) was often observed, magnetite (g=9) once, and usually small amounts of a common bacterial decay product. The latter was detected for at least 18 months film storage. ESR is a particularly good tool for observing the growth of oxic biofilms containing Fe-metabolising bacteria, and should be just as sensitive for observing Mn-metabolising bacteria in reducing conditions.
author	Whitehead,Neil Evan Tani,Atushi Kazue,Tazaki Motoji,Ikeya
spellingShingle	Whitehead,Neil Evan Tani,Atushi Kazue,Tazaki Motoji,Ikeya Preliminary results of using ESR to examine biofilms
author_facet	Whitehead,Neil Evan Tani,Atushi Kazue,Tazaki Motoji,Ikeya
author_sort	Whitehead,Neil Evan
title	Preliminary results of using ESR to examine biofilms
title_short	Preliminary results of using ESR to examine biofilms
title_full	Preliminary results of using ESR to examine biofilms
title_fullStr	Preliminary results of using ESR to examine biofilms
title_full_unstemmed	Preliminary results of using ESR to examine biofilms
title_sort	preliminary results of using esr to examine biofilms
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300011
work_keys_str_mv	AT whiteheadneilevan preliminaryresultsofusingesrtoexaminebiofilms AT taniatushi preliminaryresultsofusingesrtoexaminebiofilms AT kazuetazaki preliminaryresultsofusingesrtoexaminebiofilms AT motojiikeya preliminaryresultsofusingesrtoexaminebiofilms
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Preliminary results of using ESR to examine biofilms

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