Recombinant expression of marine shrimp lysozyme in Escherichia coli
Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study it...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2004
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300012 |
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oai:scielo:S0717-345820040003000122005-05-31Recombinant expression of marine shrimp lysozyme in Escherichia colide-la-Re-Vega,EnriqueGarcía-Orozco,Karina DCalderón-Arredondo,Sergio ARomo-Figueroa,María GabrielaIslas-Osuna,María A.Yepiz-Plascencia,Gloria M.Sotelo-Mundo,Rogerio R. Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study its function and biochemical properties we have overexpressed Lysozyme from marine shrimp (Penaeus vannamei) in E. coli. A bacterial protein expression system based on the T7 polymerase promoter was used. Although Lyz was produced as insoluble protein in inclusion bodies, its refolding led to an active protein with a yield of ~10%. Details of the protein recombinant expression techniques applied to this shrimp protein are presentedinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.7 n.3 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300012en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| description |
Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study its function and biochemical properties we have overexpressed Lysozyme from marine shrimp (Penaeus vannamei) in E. coli. A bacterial protein expression system based on the T7 polymerase promoter was used. Although Lyz was produced as insoluble protein in inclusion bodies, its refolding led to an active protein with a yield of ~10%. Details of the protein recombinant expression techniques applied to this shrimp protein are presented |
| author |
de-la-Re-Vega,Enrique García-Orozco,Karina D Calderón-Arredondo,Sergio A Romo-Figueroa,María Gabriela Islas-Osuna,María A. Yepiz-Plascencia,Gloria M. Sotelo-Mundo,Rogerio R. |
| spellingShingle |
de-la-Re-Vega,Enrique García-Orozco,Karina D Calderón-Arredondo,Sergio A Romo-Figueroa,María Gabriela Islas-Osuna,María A. Yepiz-Plascencia,Gloria M. Sotelo-Mundo,Rogerio R. Recombinant expression of marine shrimp lysozyme in Escherichia coli |
| author_facet |
de-la-Re-Vega,Enrique García-Orozco,Karina D Calderón-Arredondo,Sergio A Romo-Figueroa,María Gabriela Islas-Osuna,María A. Yepiz-Plascencia,Gloria M. Sotelo-Mundo,Rogerio R. |
| author_sort |
de-la-Re-Vega,Enrique |
| title |
Recombinant expression of marine shrimp lysozyme in Escherichia coli |
| title_short |
Recombinant expression of marine shrimp lysozyme in Escherichia coli |
| title_full |
Recombinant expression of marine shrimp lysozyme in Escherichia coli |
| title_fullStr |
Recombinant expression of marine shrimp lysozyme in Escherichia coli |
| title_full_unstemmed |
Recombinant expression of marine shrimp lysozyme in Escherichia coli |
| title_sort |
recombinant expression of marine shrimp lysozyme in escherichia coli |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2004 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300012 |
| work_keys_str_mv |
AT delarevegaenrique recombinantexpressionofmarineshrimplysozymeinescherichiacoli AT garciaorozcokarinad recombinantexpressionofmarineshrimplysozymeinescherichiacoli AT calderonarredondosergioa recombinantexpressionofmarineshrimplysozymeinescherichiacoli AT romofigueroamariagabriela recombinantexpressionofmarineshrimplysozymeinescherichiacoli AT islasosunamariaa recombinantexpressionofmarineshrimplysozymeinescherichiacoli AT yepizplascenciagloriam recombinantexpressionofmarineshrimplysozymeinescherichiacoli AT sotelomundorogerior recombinantexpressionofmarineshrimplysozymeinescherichiacoli |
| _version_ |
1718441727008702464 |